Wang Huan, Li Jing, Liu Yan-Chun, Chen Xi, Chai Tie
Department of Hematology, Tangshan Worker's Hospital, Tangshan 063000, Hebei Province, China.
Department of Hematology, Tangshan Worker's Hospital, Tangshan 063000, Hebei Province, China. E-mail:
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2018 Jun;26(3):829-835. doi: 10.7534/j.issn.1009-2137.2018.03.033.
To study the mechanism and clinical value of miR-373 in multiple myeloma.
The expressions of miR-373 in multiple myeloma cells and normal plasma cells were detected by RT-PCR, and the biological function of miR-373 in tumor was analyzed by MTT assay, flow cytometry, luciferase experiment and tumorgenesis experiment.
The miR-373 expression levels in MM patients and multiple myeloma cell lines (H929, MM1S and U266) were significantly lower than that in normal plasma cells detected by using RT-PCR (P<0.05). The proliferations of U266 and H929 cells transfected with miR-373 were significantly suppressed (P<0.05); the cell cycle of H929 cell transfected with miR-373 was arrested in the G/G phase(P<0.05) and the cell apoptosis was induced (P<0.05). Luciferase experiment revealed that miR-373 could significantly inhibit the expression of FOXM1 (P<0.05). In mouse tumorigenesis experiments, overexpression of miR-373 significantly inhibited tumor growth by decreasing FOXM1 levels (P<0.05).
miR-373 inhibits tumor growth in MM by direct targeting FOXM1, thus miR-373 shows an important clinical significance for the treatment of MM.
研究miR-373在多发性骨髓瘤中的作用机制及临床价值。
采用RT-PCR检测miR-373在多发性骨髓瘤细胞和正常浆细胞中的表达情况，通过MTT法、流式细胞术、荧光素酶实验及成瘤实验分析miR-373在肿瘤中的生物学功能。
应用RT-PCR检测发现，MM患者及多发性骨髓瘤细胞系（H929、MM1S和U266）中miR-373的表达水平显著低于正常浆细胞（P<0.05）。转染miR-373后，U266和H929细胞的增殖受到显著抑制（P<0.05）；转染miR-373的H929细胞周期阻滞于G/G期（P<0.05），并诱导细胞凋亡（P<0.05）。荧光素酶实验显示，miR-373可显著抑制FOXM1的表达（P<0.05）。在小鼠成瘤实验中，miR-373过表达通过降低FOXM1水平显著抑制肿瘤生长（P<0.05）。
miR-373通过直接靶向FOXM1抑制MM肿瘤生长，因此miR-373对MM的治疗具有重要临床意义。
