Department of Physiology, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia.
Al-Ma'arif Nursing Academy, Baturaja, South Sumatra, Indonesia.
Biol Trace Elem Res. 2019 Apr;188(2):424-433. doi: 10.1007/s12011-018-1420-0. Epub 2018 Jun 27.
Fluoride is frequently added to drinking water supplies, various food products, toothpaste, and mouth rinses to prevent tooth damage. However, at high concentrations, fluoride can cause fluorosis and damage to the brain tissue due to its excitotoxicity and oxidative stress effects. The damage of the Purkinje cells of the cerebellum can lead to motor coordination disorders. The present study aimed at investigating the effects of sodium fluoride on the motor coordination and the number of Purkinje cells of the cerebellum of rats. Adult male Wistar rats were divided into four groups, namely a control group which received reverse osmosis distilled water and three treated groups which received sodium fluoride at doses of 5, 10, or 20 mg/kg bw. The treatment lasted for 30 days. The motor coordination of the rats was examined using a rotarod prior and subsequent to the treatments. The number of Purkinje cells was estimated using physical fractionator design. The numbers of Purkinje cells of the F10 and F20 groups were significantly lower than that of the control group. No significant differences in the results of the motor coordination test were found. The administration of sodium fluoride at doses of 10 and 20 mg/kg bw caused a decrease in the number of Purkinje cells of the cerebellum in rats.
氟化物经常被添加到饮用水供应、各种食品、牙膏和漱口水中，以预防牙齿损伤。然而，在高浓度下，氟化物由于其兴奋性毒性和氧化应激作用，会导致氟中毒和脑组织损伤。小脑浦肯野细胞的损伤会导致运动协调障碍。本研究旨在探讨氟化钠对大鼠运动协调和小脑浦肯野细胞数量的影响。成年雄性 Wistar 大鼠分为四组，即对照组接受反渗透蒸馏水，三组实验组分别接受 5、10 或 20mg/kg bw 的氟化钠。治疗持续 30 天。在治疗前后，使用转棒试验检查大鼠的运动协调能力。使用物理分馏设计估计浦肯野细胞的数量。F10 和 F20 组的浦肯野细胞数量明显低于对照组。运动协调测试结果无显著差异。10 和 20mg/kg bw 的氟化钠给药剂量导致大鼠小脑浦肯野细胞数量减少。
