Suryanti S, Partadiredja G, Atthobari J
Bratisl Lek Listy. 2015;116(2):109-14. doi: 10.4149/bll_2015_021.
The present study is aimed at investigating the possible protective effects of H. sabdariffa on ethanol-elicited deficits of motor coordination and estimated total number of the Purkinje cells of the cerebellums of adolescent male Wistar rats.
Forty male Wistar rats aged 21 days were divided into five groups. Na/wtr group was given water orally and injected with normal saline intra peritoneally (ip). Eth/wtr group was given water orally and ethanol (ip). Another three experimental groups (Eth/Hsab) were given different dosages of H. sabdariffa and ethanol (ip). All groups were treated intermittently for the total period of treatment of two weeks. The motor coordination of rats was tested prior and subsequent to the treatments. The rats were euthanized, and their cerebellums were examined. The total number of Purkinje cells was estimated using physical fractionator method.
Upon revolving drum test, the number of falls of rats increased following ethanol treatment. There was no significant difference between the total number of falls prior and subsequent to treatment in all Eth/Hsab groups. The estimated total number of Purkinje cells in Eth/Hsab groups was higher than in Eth/wtr group.
H. sabdariffa may prevent the ethanol-induced deficits of motor coordination and estimated total number of Purkinje cells of the cerebellums in adolescent rats (Tab. 3, Fig. 1, Ref. 42).
本研究旨在探讨玫瑰茄对青春期雄性Wistar大鼠乙醇诱发的运动协调功能缺陷以及小脑浦肯野细胞估计总数的可能保护作用。
将40只21日龄的雄性Wistar大鼠分为五组。钠/水组经口给予水，并腹腔注射生理盐水。乙醇/水组经口给予水，并腹腔注射乙醇。另外三个实验组（乙醇/玫瑰茄组）给予不同剂量的玫瑰茄和乙醇（腹腔注射）。所有组均间歇性治疗两周。在治疗前后对大鼠的运动协调性进行测试。对大鼠实施安乐死后，检查其小脑。使用物理分割器方法估计浦肯野细胞的总数。
在转鼓试验中，乙醇处理后大鼠跌倒次数增加。所有乙醇/玫瑰茄组治疗前后的跌倒总数之间无显著差异。乙醇/玫瑰茄组浦肯野细胞的估计总数高于乙醇/水组。
玫瑰茄可能预防青春期大鼠乙醇诱导的运动协调功能缺陷以及小脑浦肯野细胞估计总数的减少（表3，图1，参考文献42）。
