在癫痫发作大鼠模型中使用化学交换饱和转移成像进行肌酸的体内定位和定量。
In Vivo Mapping and Quantification of Creatine Using Chemical Exchange Saturation Transfer Imaging in Rat Models of Epileptic Seizure.
机构信息
Faculty of Health Sciences and Brain & Mind Centre, The University of Sydney, Sydney, NSW, Australia.
Center for Bioimaging of New Drug Development, and MR Core Laboratory, Asan Medical Center, Asan Institute for Life Sciences, Seoul, Republic of Korea.
出版信息
Mol Imaging Biol. 2019 Apr;21(2):232-239. doi: 10.1007/s11307-018-1243-6.
PURPOSE
To evaluate signal changes in the hippocampus of epileptic seizure rat models, based on quantified creatine chemical exchange saturation transfer (CrCEST) signals.
PROCEDURES
CEST data and H magnetic resonance spectroscopy (H MRS) data were obtained for the two imaging groups: control (CTRL) and epileptic seizure-induced (ES; via kainic acid [KA] injection) groups. CrCEST signals in the hippocampal regions were quantitatively evaluated; correlations between CrCEST signals and phosphocreatine (PCr) and total creatine (tCr; PCr + Cr) concentrations, derived from the analysis of H MRS data, were investigated as a function of time changes (before KA injection, 3 and 5 h after KA injection).
RESULTS
Measured CrCEST signals were exhibited significant differences between before and after KA injection in the ES group. At each time point, CrCEST signals showed significant correlations with PCr concentration (all |r| > 0.59; all P < 0.05); no significant correlations were found between CrCEST signals and tCr concentrations (all |r| < 0.22; all P > 0.05).
CONCLUSIONS
CrCEST can adequately detect changes in the concentration of Cr as a result of energy metabolism, and may serve as a potentially useful tool for diagnosis and assessment of prognosis in epilepsy.
目的
基于定量肌酸化学交换饱和转移（CrCEST）信号，评估癫痫发作大鼠模型中海马的信号变化。
方法
对两组成像组（CTRL 和癫痫发作诱导组（通过注射海人酸[KA]））获得 CEST 数据和 H 磁共振波谱（H MRS）数据。对海马区的 CrCEST 信号进行定量评估；作为时间变化的函数（在 KA 注射前、KA 注射后 3 和 5 小时），研究 CrCEST 信号与来自 H MRS 数据分析的磷酸肌酸（PCr）和总肌酸（tCr；PCr+Cr）浓度之间的相关性。
结果
在 ES 组中，在 KA 注射前后测量的 CrCEST 信号存在显著差异。在每个时间点，CrCEST 信号与 PCr 浓度均显示出显著相关性（所有 |r|>0.59；所有 P<0.05）；CrCEST 信号与 tCr 浓度之间未发现显著相关性（所有 |r|<0.22；所有 P>0.05）。
结论
CrCEST 可以充分检测能量代谢导致的 Cr 浓度变化，可能成为癫痫诊断和预后评估的一种有用工具。
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