Nomura M, Imai M, Tsuda F, Furuta S, Akahane Y, Tachibana K, Usuda S, Miyakawa Y, Mayumi M
Gastroenterology. 1985 Nov;89(5):1109-13. doi: 10.1016/0016-5085(85)90217-3.
Antibody to hepatitis B core antigen of immunoglobulin A class was determined in the serum of patients infected with hepatitis B virus by a sandwich-type solid-phase radioimmunoassay with monoclonal antibodies. The antibody, as defined by a sample to normal ratio greater than 2.1, was detected in all of 39 patients with acute hepatitis, with titers varying widely depending on the time of blood sampling. In persons with persistent infection, the antibody was detected in only 2 (4%) of 46 asymptomatic carriers of the virus, contrasting with the positivity in as many as 15 (41%) of 37 patients with chronic persistent hepatitis, in 45 (94%) of 48 patients with chronic active hepatitis, and in 40 (87%) of 46 patients with liver cirrhosis with or without hepatocellular carcinoma. The mean +/- SE titer of antibody in chronic persistent hepatitis (3.8 +/- 0.9) was significantly lower than those in chronic active hepatitis (13.8 +/- 3.2) and cirrhosis with or without carcinoma (25.6 +/- 6.1) (p less than 0.001). Based on the results obtained, the antibody may reflect hepatic injury in the persistent hepatitis B virus infection.
采用单克隆抗体夹心型固相放射免疫分析法，对乙型肝炎病毒感染患者血清中的免疫球蛋白A类乙型肝炎核心抗原抗体进行检测。以样本与正常对照比值大于2.1为标准，在39例急性肝炎患者中均检测到该抗体，其滴度根据采血时间不同差异很大。在持续性感染患者中，46例无症状病毒携带者仅2例（4%）检测到该抗体，与之形成对比的是，37例慢性持续性肝炎患者中有15例（41%）呈阳性，48例慢性活动性肝炎患者中有45例（94%）呈阳性，46例有或无肝细胞癌的肝硬化患者中有40例（87%）呈阳性。慢性持续性肝炎患者抗体的平均滴度±标准误为（3.8±0.9），显著低于慢性活动性肝炎患者（13.8±3.2）以及有或无癌的肝硬化患者（25.6±6.1）（p<0.001）。根据所得结果，该抗体可能反映持续性乙型肝炎病毒感染中的肝损伤情况。
