Tianjin Univ., China.
Shanghai Jiao Tong Univ., China.
J Biomed Opt. 2018 Jun;23(6):1-6. doi: 10.1117/1.JBO.23.6.065005.
Mitochondrial oxidative flashes (mitoflashes) are oxidative burst events in mitochondria. It is crosslinked with numerous mitochondrial molecular processes and related with pivotal cell functions such as apoptosis and respiration. In previous research, mitoflashes were found as spontaneous occasional events. It would be observed more frequently if cells were treated with proapoptotic chemicals. We show that multiple mitoflashes can be initiated by a single femtosecond-laser stimulation that was tightly focused on a diffraction-limited spot in the mitochondrial tubular structure. The mitoflash events triggered by different photostimulations are quantified and analyzed. The width and amplitude of mitoflashes are found very sensitive to stimulation parameters including laser power, exposure duration, and total incident laser energy. This study provides a quantitative investigation on the photostimulated mitoflashes. It may thus demonstrate such optical method to be a promising technique in future mitochondrial research.
线粒体氧化闪络（mitoflashes）是线粒体中的氧化爆发事件。它与许多线粒体分子过程交联，并与细胞凋亡和呼吸等关键细胞功能相关。在之前的研究中，氧化闪络被发现是自发的偶发事件。如果细胞用促凋亡化学物质处理，氧化闪络会更频繁地发生。我们表明，单个飞秒激光刺激可以在线粒体管状结构的一个衍射受限点上紧密聚焦，从而引发多个氧化闪络。我们对不同光刺激引发的氧化闪络事件进行了量化和分析。我们发现氧化闪络的宽度和幅度对刺激参数非常敏感，包括激光功率、照射持续时间和总入射激光能量。这项研究对光刺激的氧化闪络进行了定量研究。因此，它可能证明这种光学方法是未来线粒体研究中很有前途的技术。
