Yanyan Shi, Yanlei Guo, Ting Zhang, Shigang Ding
1Research Center of Clinical Epidemiology,Peking University Third Hospital,Beijing 100191,P.R.China.
2Department of Gastroenterology,Peking University Third Hospital,Beijing 100191,P.R.China.
Microsc Microanal. 2018 Jun;24(3):277-283. doi: 10.1017/S1431927618000314.
Helicobacter pylori is a major cause of gastric diseases including gastric cancer. This study was aimed to explore whether hydrotalcite can inhibit H. pylori infection of gastric epithelial cells.
the gastric epithelial cell line GES-1 and the gastric cancer cell line BGC823 were infected with H. pylori at multiplicities of infections (MOIs) of 50:1 and 100:1. Hydrotalcite was added to cell cultures. Cell apoptosis and cell cycle analysis were performed to measure the situation of cell growth. The main changes of cell ultrastructure were observed by transmission electron microscopy. H. pylori cell adhesion was observed by scanning electron microscopy.
hydrotalcite could significantly inhibit cell apoptosis of GES-1 and cell proliferation of BGC823 induced by H. pylori infection at an MOI of 50:1. Hydrotalcite treatment protected gastric cells from H. pylori infection, and H. pylori adhesion to gastric cells was reduced. However, hydrotalcite could not reverse damage induced by H. pylori infection at an MOI of 100:1.
hydrotalcite can protect gastric cells from H. pylori infection when cell damage is not serious. It can weaken the damage of cells induced by H. pylori and decrease H. pylori adhesion to gastric cells.
幽门螺杆菌是包括胃癌在内的胃部疾病的主要病因。本研究旨在探讨水滑石是否能抑制幽门螺杆菌对胃上皮细胞的感染。
将胃上皮细胞系GES-1和胃癌细胞系BGC823分别以50:1和100:1的感染复数（MOI）感染幽门螺杆菌。向细胞培养物中添加水滑石。进行细胞凋亡和细胞周期分析以检测细胞生长情况。通过透射电子显微镜观察细胞超微结构的主要变化。通过扫描电子显微镜观察幽门螺杆菌的细胞黏附情况。
水滑石能显著抑制MOI为50:1时幽门螺杆菌感染诱导的GES-1细胞凋亡和BGC823细胞增殖。水滑石处理可保护胃细胞免受幽门螺杆菌感染，并减少幽门螺杆菌对胃细胞的黏附。然而，水滑石不能逆转MOI为100:1时幽门螺杆菌感染所诱导的损伤。
当细胞损伤不严重时，水滑石可保护胃细胞免受幽门螺杆菌感染。它可减轻幽门螺杆菌对细胞的损伤，并减少幽门螺杆菌对胃细胞的黏附。
