Lin Haiyang, Zheng Minqiao, Mao Xiaojie, Feng Xuewen, Li Jing, Rao Gaofeng, Lin Fang
Department of Endocrinology, Ningbo First Hospital, No.59 Liuting Street, 315010 Ningbo, China.
Endokrynol Pol. 2018;69(4). doi: 10.5603/EP.a2018.0040. Epub 2018 Jun 28.
Oxytocin might be used therapeutically as an ally to rescue osteopathy resulting from diabetes. However, the in vivo effects of oxytocin on marrow adipogenesis in diabetes remain unknown. In this longitudinal study, we aimed to investigate the protective ef-fects of oxytocin on diabetes-induced marrow adiposity in rabbits using proton MR spectroscopy.
Forty-five female New Zealand rabbits were randomly divided into controls, diabetes, and diabetes treated with oxytocin (ip, 0.78 mg/kg) for six months. Marrow fat fraction (FF) was determined by proton MR spectroscopy at baseline, and at three and six months. Bone mineral density was measured by dual-energy X-ray absorptiometry. Serum biomarkers, glycolipid metabolism, and histological analysis of marrow adipocytes were determined.
Oxytocin treatment had positive metabolic effects in diabetic rabbits, which was based on the changes in glucose metabolism, insulin sensitivity, and lipid profiles. The diabetic rabbits demonstrated dramatic marrow adiposity in a time-dependent manner; at three and six months the FF percentage changes from baseline were 10.1% and 25.8%, respectively (all P < 0.001). Moreover, oxytocin treatment significantly reversed FF values and quantitative parameters of marrow adipocyte in diabetic rabbits to levels of naive control rabbits. Oxytocin improved bone formation marker in diabetic rabbits compared to the saline group. Also, treatment of diabetic rabbits with oxytocin significantly mitigated bone deterioration when compared with the saline-treated diabetic group (all P < 0.05).
Oxytocin appears to alleviate harmful effects of hyperglycaemia on marrow adiposity. Proton MR spectroscopy may be a valuable tool, providing complementary information on efficacy assessments.
催产素可能作为一种辅助治疗手段用于挽救糖尿病所致的骨病。然而，催产素对糖尿病状态下骨髓脂肪生成的体内作用仍不清楚。在这项纵向研究中，我们旨在利用质子磁共振波谱研究催产素对兔糖尿病诱导的骨髓脂肪增多的保护作用。
45只雌性新西兰兔被随机分为对照组、糖尿病组和接受催产素治疗（腹腔注射，0.78mg/kg）的糖尿病组，为期6个月。在基线、3个月和6个月时通过质子磁共振波谱测定骨髓脂肪分数（FF）。采用双能X线吸收法测量骨密度。测定血清生物标志物、糖脂代谢以及骨髓脂肪细胞的组织学分析。
催产素治疗对糖尿病兔有积极的代谢作用，这基于葡萄糖代谢、胰岛素敏感性和血脂谱的变化。糖尿病兔呈现出随时间推移显著的骨髓脂肪增多；在3个月和6个月时，FF百分比相对于基线的变化分别为10.1%和25.8%（均P<0.001）。此外，催产素治疗显著将糖尿病兔的FF值和骨髓脂肪细胞的定量参数逆转至未处理对照兔的水平。与生理盐水组相比，催产素改善了糖尿病兔的骨形成标志物。而且，与生理盐水治疗的糖尿病组相比，用催产素治疗糖尿病兔显著减轻了骨质恶化（均P<0.05）。
催产素似乎可减轻高血糖对骨髓脂肪增多的有害影响。质子磁共振波谱可能是一种有价值的工具，可为疗效评估提供补充信息。
