Velegzhaninov Ilya O, Ermakova Anastasia V, Klokov Dmitry Yu
a Department of Radioecology , Institute of Biology of Komi Science Centre of Ural Branch of RAS , Syctyvkar , Russia.
b Polytechnical Institute, Vyatka State University , Kirov , Russia.
Int J Radiat Biol. 2018 Sep;94(9):825-828. doi: 10.1080/09553002.2018.1492167. Epub 2018 Aug 21.
Exposure to high dose ionizing radiation leads to premature cell senescence and suppression of cell proliferation. In contrast, low dose and low dose-rate gamma-irradiation can lead to stimulation of cell proliferation. We aimed to examine whether the low dose radiation-induced proliferation of normal human fibroblasts can lead to a progressive depletion of proliferation potential and to an early onset of senescence.
Normal human embryonic lung fibroblasts (HELF-104) at passage 22-24 were gamma-irradiated with doses of 0 (sham-irradiation), 10, 30, 50, 90, 120, 150, 200, and 500 mGy as well as 1 and 2 Gy. After irradiation, the fraction of cells positively stained for senescence-associated β-galactosidase activity was measured weekly until the cell culture completely ceased to proliferate.
We show that single irradiation of HELF-104 cells with 30 and 50 mGy resulted in deceleration of senescence. The suppression of senescence was observed during almost the entire length of the study up to a complete arrest of cell growth.
Our data, together with the previously published observation of delayed stimulation of proliferation in HELF-104 cells exposed to 30 mGy, suggest that low dose gamma-irradiation can increase the overall proliferative potential of normal human fibroblasts.
暴露于高剂量电离辐射会导致细胞过早衰老并抑制细胞增殖。相比之下，低剂量和低剂量率的伽马辐射可导致细胞增殖受到刺激。我们旨在研究低剂量辐射诱导的正常人成纤维细胞增殖是否会导致增殖潜能的逐渐耗尽和衰老的提前发生。
对传代22 - 24代的正常人胚肺成纤维细胞（HELF - 104）进行伽马辐射，剂量分别为0（假辐射）、10、30、50、90、120、150、200和500毫戈瑞以及1和2戈瑞。辐射后，每周测量衰老相关β - 半乳糖苷酶活性呈阳性染色的细胞比例，直至细胞培养完全停止增殖。
我们发现，用30和50毫戈瑞对HELF - 104细胞进行单次辐射会导致衰老减速。在几乎整个研究期间直至细胞生长完全停止，均观察到衰老受到抑制。
我们的数据，连同先前发表的关于暴露于30毫戈瑞的HELF - 104细胞增殖延迟刺激的观察结果，表明低剂量伽马辐射可增加正常人成纤维细胞的总体增殖潜能。
