Lecis Roberta, Secci Fabio, Antuofermo Elisabetta, Nuvoli Sara, Cacciotto Carla, Pittau Marco, Alberti Alberto
1 Department of Veterinary Medicine, University of Sassari, Via Vienna 2, 07100, Sassari, Italy.
2 Wildlife Recovery Centre/Veterinary Clinic, Via Europa 19, 09010, Villamassargia, Cagliari, Italy.
J Wildl Dis. 2019 Jan;55(1):142-148. doi: 10.7589/2017-10-265. Epub 2018 Jun 28.
Poxvirus infections have been reported in domestic, captive, and wild avian hosts including many raptor species. A wild Common Buzzard ( Buteo buteo) admitted to a wildlife veterinary clinic in Sardinia, Italy, showed multiple, wart-like proliferative cutaneous lesions on both legs. Histologically, there was ballooning degeneration and large intracytoplasmic inclusion bodies consistent with avipoxvirus (APV) infection. Diagnosis was confirmed by PCR detecting APV genes: P4b (locus fpv167), P35 (locus fpv140), and partial DNA polymerase. Phylogenetic analyses were performed to compare the detected virus with a panel of selected APVs. Analyses of P4b and DNA polymerase assigned the virus to clade A (fowlpox virus), subclade A7, grouping with many other APVs previously isolated in birds of prey. Further research should highlight the diversity of avian pox viral strains circulating among Common Buzzards as well as the phylogenetic role of locus fpv140 (P35) in comparison with the more-conserved P4b and DNA polymerase genes.
痘病毒感染已在包括许多猛禽物种在内的家养、圈养和野生禽类宿主中被报道。一只被送往意大利撒丁岛一家野生动物兽医诊所的野生普通鵟（Buteo buteo）双腿出现多处疣状增生性皮肤病变。组织学检查显示，存在气球样变性和大的胞质内包涵体，符合禽痘病毒（APV）感染。通过PCR检测APV基因：P4b（基因座fpv167）、P35（基因座fpv140）和部分DNA聚合酶，确诊了该病。进行了系统发育分析，以将检测到的病毒与一组选定的APV进行比较。对P4b和DNA聚合酶的分析将该病毒归为A分支（禽痘病毒），A7亚分支，与之前在猛禽中分离出的许多其他APV归为一组。进一步的研究应突出普通鵟中传播的禽痘病毒株的多样性，以及与更保守的P4b和DNA聚合酶基因相比，基因座fpv140（P35）的系统发育作用。
