Department of Urology, Ninghai First Hospital, Zhejiang, China.
Department of Urology, Ninghai Hospital, Branch of Shanghai Tenth People's Hospital, Zhejiang, China.
J Cell Physiol. 2018 Dec;233(12):9611-9619. doi: 10.1002/jcp.26864. Epub 2018 Jun 28.
Recently, increasing studies showed that long noncoding RNAs (lncRNAs) play critical roles in tumor progression. However, the function and underlying mechanism of HOMEOBOX A11 antisense RNA (HOXA11-AS) on renal cancer remain unclear. In the current study, our data showed that the expression of HOXA11-AS was significantly upregulated in clear cell renal cell carcinoma (ccRCC) tissues and cell lines. High HOXA11-AS expression was associated with the advanced clinical stage, tumor stage, and lymph node metastasis. Function assays showed that HOXA11-AS inhibition significantly suppressed renal cancer cells growth, invasion, and ETM phenotype. In addition, underlying mechanism revealed that HOXA11-AS could act as a competing endogenous RNA (ceRNA) that repressed miR-146b-5p expression, which regulated its downstream target MMP16 in renal cancer. Taken together, our findings suggested that HOXA11-AS could promote renal cancer cells growth and invasion by modulating miR-146b-5p-MMP16 axis. Thus, our findings suggested that HOXA11-AS could serve as potential therapeutic target for the treatment of renal cancer.
最近，越来越多的研究表明长非编码 RNA（lncRNA）在肿瘤进展中发挥着关键作用。然而，HOXA11 反义 RNA（HOXA11-AS）在肾细胞癌中的功能和潜在机制尚不清楚。在本研究中，我们的数据表明 HOXA11-AS 在透明细胞肾细胞癌（ccRCC）组织和细胞系中的表达显著上调。高 HOXA11-AS 表达与晚期临床分期、肿瘤分期和淋巴结转移有关。功能分析表明，HOXA11-AS 抑制显著抑制肾癌细胞的生长、侵袭和上皮间质转化表型。此外，潜在机制表明 HOXA11-AS 可以作为竞争性内源性 RNA（ceRNA），抑制 miR-146b-5p 的表达，从而调节肾癌细胞中的下游靶基因 MMP16。综上所述，我们的研究结果表明，HOXA11-AS 可以通过调节 miR-146b-5p-MMP16 轴促进肾癌细胞的生长和侵袭。因此，我们的研究结果表明，HOXA11-AS 可以作为治疗肾细胞癌的潜在治疗靶点。
