Liu H, Zhao J, Lv J
Eur J Gynaecol Oncol. 2017;38(2):236-240.
microRNAs (miRNAs), which can regulate cell biological behaviors such as proliferation and apoptosis as oncogenes or anti-oncogenes, are closely associated with cancer onset and progression. The aim of this study was to detect the expres- sion changes of miR-101 in cervical cancer tissues and the effects on the biological functions of cervical cancer SiHa cells.
Through transient transfection of SiHa cells with mature miR-101 sequences, the effects on apoptosis, proliferation, and cell cycle were evaluated by real-time PCR, CCK-8 assay, and flow cytometry.
Significantly less miR-101 was expressed in cervical cancer tissues than that in normal cervical tissues. miR-101 was overexpressed in SiHa cells through transient transfection of miR-101 mimics. CCK-8 assay and flow cytometry showed that miR-101 overexpression significantly inhibited cell proliferation, pro- moted apoptosis, and arrested them in the G(l)/S phase. Real-time PCR exhibited that Mcl-i and c-Fos mRNA expressions significantly decreased.
miR-101 significantly reduced the viability of SiHa cells as a potential anti-oncogene.
微小RNA（miRNA）作为癌基因或抑癌基因可调节细胞增殖和凋亡等生物学行为，与癌症的发生和发展密切相关。本研究旨在检测miR-101在宫颈癌组织中的表达变化及其对宫颈癌SiHa细胞生物学功能的影响。
通过将成熟miR-101序列瞬时转染SiHa细胞，采用实时PCR、CCK-8法和流式细胞术评估其对凋亡、增殖和细胞周期的影响。
宫颈癌组织中miR-101的表达明显低于正常宫颈组织。通过瞬时转染miR-101模拟物使SiHa细胞中miR-101过表达。CCK-8法和流式细胞术显示，miR-101过表达显著抑制细胞增殖，促进凋亡，并使细胞停滞于G(1)/S期。实时PCR显示Mcl-i和c-Fos mRNA表达显著降低。
miR-101作为一种潜在的抑癌基因可显著降低SiHa细胞的活力。
