生长研究中使用颜色替代大小的方法

The Use of Colors as an Alternative to Size in Growth Studies.

作者信息

Cambaza Edgar, Koseki Shigenobu, Kawamura Shuso

机构信息

Laboratory of Food Process Engineering, Graduate School of Agriculture, Hokkaido University, Sapporo 060-0808, Hokkaido, Japan.

Department of Biological Sciences, Faculty of Sciences, Eduardo Mondlane University, Av. Julius Nyerere, nr. 3453 Maputo, Moçambique.

出版信息

Foods. 2018 Jun 27;7(7):100. doi: 10.3390/foods7070100.

DOI:10.3390/foods7070100

PMID:29954087

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6069261/

Abstract

Size-based fungal growth studies have limitations. For example, the growth in size stops in closed systems once it reaches the borders and poorly describes metabolic status, especially in the stationary phase. This might lead mycotoxin studies to unrealistic results. Color change could be a viable alternative, as pigments result from a mold’s metabolic activity. This study aimed to verify the possibility of using gray values and the RGB system to analyze the growth of . It consisted of color and area measurements using ImageJ software for specimens grown in yeast extract agar (YEA). The results suggest the utility of color and gray values as reliable tools to analyze the growth of .

摘要

基于大小的真菌生长研究存在局限性。例如，在封闭系统中，一旦生长达到边界，大小增长就会停止，而且它很难描述代谢状态，尤其是在稳定期。这可能会导致霉菌毒素研究得出不切实际的结果。颜色变化可能是一种可行的替代方法，因为色素是霉菌代谢活动的产物。本研究旨在验证使用灰度值和RGB系统分析……生长的可能性。它包括使用ImageJ软件对在酵母提取物琼脂（YEA）上生长的标本进行颜色和面积测量。结果表明，颜色和灰度值作为分析……生长的可靠工具具有实用性。