Department of Crop Science, Chungnam National University, 99 Daehak-Ro, Yuseong-gu, Daejeon 34134, Korea.
National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun, Jeonbuk 54875, Korea.
Molecules. 2018 Jun 27;23(7):1558. doi: 10.3390/molecules23071558.
A total of seven phenolics and 44 metabolites was profiled in white flowers of and violet flowers of using high-performance liquid chromatography (HPLC), electrospray ionization-mass spectrometry (ESI-MS), and gas chromatography time-of-flight mass spectrometry (GC-TOFMS). Seven phenylpropanoid compounds were identified in white flowers by liquid chromatography mass spectrometry (LC-MS). An HPLC analysis showed that phenylpropanoid accumulation in violet flowers was 1.48 times higher than that in white flowers. Furthermore, superoxide dismutase (SOD)-like activity and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity were determined to investigate the antioxidant properties of secondary metabolites in different flowers. Violet flowers showed higher SOD-like and DPPH activity than white flowers. In addition, anti-inflammatory activity measured using a nitric oxide assay was higher in violet flowers than in white flowers. Our results provide valuable information on the relationship between primary and secondary metabolites, and synergistic antioxidant and anti-inflammatory properties derived from phenolic compounds in different colored flowers.
利用高效液相色谱法（HPLC）、电喷雾电离质谱（ESI-MS）和气相色谱飞行时间质谱（GC-TOFMS），对白花和紫花中的七种酚类化合物和 44 种代谢产物进行了分析。通过液相色谱质谱（LC-MS）鉴定了白花中的七种苯丙素类化合物。HPLC 分析表明，紫花中苯丙素类化合物的积累量比白花高 1.48 倍。此外，测定了超氧化物歧化酶（SOD）样活性和 1,1-二苯基-2-苦基肼基（DPPH）自由基清除活性，以研究不同花色中次生代谢物的抗氧化特性。紫花的 SOD 样活性和 DPPH 活性均高于白花。此外，用一氧化氮测定法测定的抗炎活性在紫花中也高于白花。我们的研究结果为不同花色中初级和次级代谢物之间的关系以及酚类化合物协同抗氧化和抗炎特性提供了有价值的信息。
