Raileanu Alexandra, Piazzoni Claudio, Borghi Francesca, Bettini Luca Giacomo, Shacham-Diamand Yosi, Santaniello Tommaso, Milani Paolo
J Nanosci Nanotechnol. 2018 Oct 1;18(10):6905-6912. doi: 10.1166/jnn.2018.15518.
Here we report on the fabrication and characterization of cluster-assembled nanostructured zirconia electrodes for the electrochemical detection of enzymatically produced thiocholine. Zirconia nanostructures are produced by Supersonic Cluster Beam Deposition on thin gold films. This technique enables nanoscale control of the deposited film surface morphology, providing high active surface area for electrochemical detection of the analyte, along with high double-layer capacitance and suitable charge transfer resistance of the system. The electrochemical behavior of the electrodes has been characterized in the presence of the Potassium ferricyanide/Potassium ferrocyanide redox couple and the system performance showed to be enhanced starting from a thickness of the deposited layer of 60 nm. The electrochemical response for the oxidation of an enzymatic product was assessed by means of cyclic voltammetry, electrochemical impedance spectroscopy and chronoamperometry. The nanostructured zirconia film yields a good electrochemical detection of thiocholine. The limit of detection for thiocholine under working potential of 0.810-0.820 V versus reference was found to be comprised between 0.25 μM and 1.3 μM. Nanostructured electrodes, combining gold and zirconia nanoparticles can be implemented as functional transducers in biosensing devices, for example based on Acetylcholinesterase for electrochemical detection of polluting agents.
在此，我们报告用于酶促产生的硫代胆碱电化学检测的团簇组装纳米结构氧化锆电极的制备与表征。氧化锆纳米结构通过超声团簇束沉积法在薄金膜上制备。该技术能够对沉积膜的表面形态进行纳米级控制，为分析物的电化学检测提供高活性表面积，同时具有高双层电容和系统合适的电荷转移电阻。在铁氰化钾/亚铁氰化钾氧化还原对存在的情况下对电极的电化学行为进行了表征，并且系统性能从沉积层厚度为60 nm开始显示出增强。通过循环伏安法、电化学阻抗谱和计时电流法评估了酶促产物氧化的电化学响应。纳米结构氧化锆膜对硫代胆碱产生了良好的电化学检测效果。相对于参比电极，在工作电位为0.810 - 0.820 V时，硫代胆碱的检测限在0.25 μM至1.3 μM之间。结合金和氧化锆纳米颗粒的纳米结构电极可作为生物传感装置中的功能换能器，例如基于乙酰胆碱酯酶用于污染物的电化学检测。
