Zhou Xiaolai, Sullivan Peter M, Paushter Daniel H, Hu Fenghua
Department of Molecular Biology and Genetics, Weill Institute for Cell and Molecular Biology, Cornell University, Ithaca, NY, USA.
Methods Mol Biol. 2018;1806:269-288. doi: 10.1007/978-1-4939-8559-3_18.
Accumulating evidence suggests that progranulin is essential for proper lysosomal function. Progranulin is a lysosomal resident protein and sortilin has been demonstrated to be the lysosomal trafficking receptor for progranulin. Here we describe the methods used to study the interaction between progranulin and sortilin, as well as the critical role of sortilin in mediating the lysosomal delivery of progranulin.
越来越多的证据表明，颗粒蛋白前体对于正常的溶酶体功能至关重要。颗粒蛋白前体是一种溶酶体驻留蛋白，而sortilin已被证明是颗粒蛋白前体的溶酶体转运受体。在此，我们描述了用于研究颗粒蛋白前体与sortilin之间相互作用的方法，以及sortilin在介导颗粒蛋白前体向溶酶体转运中的关键作用。
