Yue Yongfei, Xu Duo, Wang Yun, Wang Xiaoyan, Xia Fei
Department of Obstetrics and Gynecology, The First Affiliated Hospital of SooChow University, Suzhou, China.
Department of Obstetrics and Gynecology, Suzhou Municipal Hospital Affiliated to Nanjing Medical University, Suzhou, China.
J Obstet Gynaecol Res. 2018 Aug;44(8):1377-1383. doi: 10.1111/jog.13681. Epub 2018 Jun 28.
To analyze inducible nitric oxide synthase (iNOS) and neuropeptide Y (NPY) expression in maternal plasma and placentas of human with intrahepatic cholestasis of pregnancy (ICP).
The plasma and placentas were collected from 35 women with normal pregnancies and 33 women with ICP. Enzyme-linked immunosorbent assays were used to investigate maternal plasma iNOS and NPY levels. The mRNA levels and cell-specific localization of iNOS and NPY were determined by quantitative PCR, Western Blotting and immunohistochemical analysis in placentas.
In human placentas, it revealed iNOS and NPY were mainly localized in syncytiotrophoblast, cytotrophoblastin and vascular endothelium cells using immunohistochemistry analysis. iNOS protein and mRNA expression in ICP maternal plasma and placental tissue were significantly lower than in control groups (P <0.01). In maternal plasma and placentas tissue from ICP patients, a marked up-regulation of NPY protein and mRNA expression were observed (P <0.01).
iNOS and NPY may play a role in the effect of maternal cholestasis on the placenta. The down-regulation of iNOS and up-regulation of NPY in ICP may influence the blood flow of the utero-placental-fetal unit, which may play a significant role in poor fetoplacental vascular perfusion, acute hypoxia and adverse pregnancy outcomes.
分析妊娠肝内胆汁淤积症（ICP）患者母体血浆和胎盘组织中诱导型一氧化氮合酶（iNOS）和神经肽Y（NPY）的表达情况。
收集35例正常妊娠妇女和33例ICP患者的血浆及胎盘组织。采用酶联免疫吸附测定法检测母体血浆中iNOS和NPY水平。通过定量PCR、蛋白质免疫印迹法及免疫组织化学分析法测定胎盘组织中iNOS和NPY的mRNA水平及细胞特异性定位。
免疫组织化学分析显示，在人胎盘组织中，iNOS和NPY主要定位于合体滋养层细胞、细胞滋养层细胞及血管内皮细胞。ICP患者母体血浆和胎盘组织中iNOS蛋白及mRNA表达水平显著低于对照组（P<0.01）。而在ICP患者的母体血浆和胎盘组织中，NPY蛋白及mRNA表达水平显著上调（P<0.01）。
iNOS和NPY可能在母体胆汁淤积对胎盘的影响中发挥作用。ICP患者中iNOS的下调和NPY的上调可能影响子宫 - 胎盘 - 胎儿单元的血流，这可能在胎盘血管灌注不良、急性缺氧及不良妊娠结局中起重要作用。
