Institute of Applied Physics RAS, Nizhny Novgorod, Russia.
Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russia.
J Biomed Opt. 2018 Jun;23(9):1-9. doi: 10.1117/1.JBO.23.9.091412.
Employment of chlorin-based photosensitizers (PSs) provides additional advantages to photodynamic therapy (PDT) due to absorption peak around 405 nm allowing for superficial impact and efficient antimicrobial therapy. We report on the morphological and clinical study of the efficiency of PDT at 405 nm employing chlorin-based PS. Numerical studies demonstrated difference in the distribution of absorbed dose at 405 nm in comparison with traditionally employed wavelength of 660 nm and difference in the in-depth absorbed dose distribution for skin and mucous tissues. Morphological study was performed at the inner surface of rabbit ear with histological examinations at different periods after PDT procedure. Animal study revealed tissue reaction to PDT consisting in edema manifested most in 3 days after the procedure and neoangiogenesis. OCT diagnostics was confirmed by histological examination. Clinical study included antimicrobial PDT of pharynx chronic inflammatory diseases. It revealed no side effects or complications of the PDT procedure. Pharyngoscopy indicated reduction of inflammatory manifestations, and, in particular cases, hypervascularization was observed. Morphological changes were also detected in the course of monitoring, which are in agreement with pharyngoscopy results. Microbiologic study after PDT revealed no pathogenic bacteria; however, in particular cases, saprophytic flora was detected.
基于氯的光敏剂 (PS) 的应用为光动力疗法 (PDT) 提供了额外的优势，因为其 405nm 的吸收峰允许进行浅表作用和有效的抗菌治疗。我们报告了基于氯的 PS 在 405nm 下进行 PDT 的形态学和临床研究效率。数值研究表明，与传统使用的 660nm 波长相比，在 405nm 处吸收剂量的分布存在差异，并且皮肤和粘膜组织的深部吸收剂量分布也存在差异。形态学研究在兔耳内表面进行，并在 PDT 后不同时期进行组织学检查。动物研究显示 PDT 引起的组织反应包括水肿，在术后 3 天最为明显，以及新血管生成。OCT 诊断通过组织学检查得到证实。临床研究包括对咽部慢性炎症性疾病进行抗菌 PDT。它没有发现 PDT 程序的副作用或并发症。咽窥镜检查显示炎症表现减轻，在某些情况下观察到血管增多。在监测过程中还检测到了形态学变化，这些变化与咽窥镜检查结果一致。PDT 后的微生物学研究未发现致病菌，但在某些情况下检测到了腐生菌群。
