Chenxinghai Hospital of Zhongshan City, No. 18 Zhuyuan Road, Xiaolan Town, Zhongshan, Guangdong, 528415, China.
Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine and Pharmacology, No. 185 Pu'an Road, Huangpu District, Shanghai, 200021, China.
Biomed Pharmacother. 2018 Oct;106:54-60. doi: 10.1016/j.biopha.2018.06.092. Epub 2018 Jun 24.
Chronic kidney disease-mineral and bone disorder (CKD-MBD) play a critical role in the pathogenesis of cardiovascular complications in patients with chronic kidney disease (CKD). Zuogui pill as a traditional Chinese herbal drug has been used for nourish kidney essence improve bone malnutrition of renal bone disease by regulating the metabolism of calcium and phosphorus and participating in osteoblast metabolism. In the present study, 5/6 nephrectomy rat model was used to reveal the mechanism of zuogui pill in treatment of CKD-MBD. Compared with sham rats, the levels of serum phosphorus, PTH, iPTH and creatinine were significantly decreased, while the serum calcium level was significantly increased, and the Cbfa1 protein level was significantly decreased and FGF23 protein level was significantly increased by Zuogui pill treatment. Compared with model rats, the BMD of rat was significantly increased by Zuogui pill treatment. Histological analysis revealed that the kidney injury of rats with CKD was significantly reduced by zuogui pill treatment. Compared with model rats, the CYP27B1 mRNA level was significantly increased, and the PTH mRNA level and NaPiIIa protein level were significantly decreased in the kidney by zuogui pill treatment. We inferred that zuogui pill exhibited potential therapeutic effects on CKD-MBD in the rats by regulating bone metabolism and nourish kidney.
慢性肾脏病-矿物质和骨异常（CKD-MBD）在慢性肾脏病（CKD）患者心血管并发症的发病机制中起关键作用。左归丸作为一种中药，已被用于通过调节钙磷代谢和参与成骨细胞代谢来滋养肾脏精华，改善肾性骨病的骨营养不良。在本研究中，使用 5/6 肾切除术大鼠模型来揭示左归丸治疗 CKD-MBD 的机制。与假手术大鼠相比，左归丸治疗后血清磷、PTH、iPTH 和肌酐水平显著降低，而血清钙水平显著升高，Cbfa1 蛋白水平显著降低，FGF23 蛋白水平显著升高。与模型大鼠相比，左归丸治疗可显著增加大鼠的骨密度。组织学分析表明，左归丸治疗可显著减轻 CKD 大鼠的肾脏损伤。与模型大鼠相比，左归丸治疗可使肾脏中的 CYP27B1mRNA 水平显著升高，PTHmRNA 水平和 NaPiIIa 蛋白水平显著降低。我们推断，左归丸通过调节骨代谢和滋养肾脏对 CKD-MBD 大鼠具有潜在的治疗作用。
