Internal Medicine of Traditional Chinese Medicine, The 4nd Clinical Medical College, Zhejiang Chinese Medical University, Zhejiang, China.
Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Zhejiang, China.
J Affect Disord. 2018 Oct 1;238:609-614. doi: 10.1016/j.jad.2018.06.034. Epub 2018 Jun 19.
Depression is a common mental disorder with unknown mechanism. Emerging evidence shows that miRNAs play a critical role in the process of depression. Here we reported the cerebrospinal fluid (CSF) miR-16 expression and its association with miR-16 and serotonin transporter (SERT) in the raphe of a rat model of depression.
20 rats were randomized to the control or CUMS (chronic unpredictable mild stress) group. The rats in the CUMS group underwent CUMS for 21 days, while those in the control group received no treatment. After anesthetization, CSF was collected for the measurement of miR-16. Then raphes from all rats were separated for determination of miR-16 and SERT protein.
The expression levels of miR-16 in CSF and raphe of the CUMS group were significantly lower than those of the control group (P = 0.007 and 0.031). However, SERT protein in raphe of the CUMS group was obviously increased as compared that of the control group (P = 0.005). There was a positive correlation between CSF miR-16 and raphe miR-16 (r = 0.95, P = 0.000). Meanwhile, negative correlations between miR-16 and SERT protein in raphe (r = -0.70 P = 0.02), between CSF miR-16 and raphe SERT protein (r = -0.86, P = 0.002) were observed in the CUMS group.
We have not explored the reason why CSF miR-16 was decreased in the rat model of depression and only tested the association of miR-16 between CSF and raphe.
CSF miR-16 was involved in the pathogenesis of depression via reflecting raphe miR-16 level, and thus affecting raphe SERT expression.
抑郁症是一种常见的精神障碍，其发病机制尚不清楚。新出现的证据表明，miRNA 在抑郁症的发生过程中起着关键作用。在这里，我们报道了抑郁大鼠模型脑脊液（CSF）miR-16 的表达及其与中缝核 miR-16 和 5-羟色胺转运体（SERT）的关系。
将 20 只大鼠随机分为对照组或 CUMS（慢性不可预测性轻度应激）组。CUMS 组大鼠接受 21 天 CUMS 处理，而对照组大鼠未接受任何处理。麻醉后收集 CSF 用于检测 miR-16。然后分离所有大鼠的中缝核以测定 miR-16 和 SERT 蛋白。
CUMS 组大鼠 CSF 和中缝核 miR-16 的表达水平明显低于对照组（P=0.007 和 0.031）。然而，CUMS 组大鼠中缝核 SERT 蛋白明显高于对照组（P=0.005）。CSF miR-16 与中缝核 miR-16 之间存在正相关（r=0.95，P=0.000）。同时，在 CUMS 组中，miR-16 与中缝核 SERT 蛋白之间存在负相关（r=-0.70，P=0.02），CSF miR-16 与中缝核 SERT 蛋白之间存在负相关（r=-0.86，P=0.002）。
我们尚未探讨 CSF miR-16 在抑郁大鼠模型中降低的原因，仅测试了 miR-16 在 CSF 和中缝核之间的相关性。
CSF miR-16 通过反映中缝核 miR-16 水平参与抑郁症的发病机制，从而影响中缝核 SERT 表达。
