链霉菌噬菌体VWB的限制性内切酶图谱。
A restriction endonuclease map of Streptomyces phage VWB.
作者信息
Anné J, Verheyen P, Volckaert G, Eyssen H
出版信息
Mol Gen Genet. 1985;200(3):506-7. doi: 10.1007/BF00425741.
PMID:2995769
Abstract
A physical map of the actinophage VWB has been constructed using the restriction endonucleases BglII, ClaI, EcoRI, EcoRV, HindIII, KpnI and SphI. Phage VWB, genome size 47.3 kb, propagates on Streptomyces venezuelae, and it can also lysogenise this species. The three BglII-generated fragments of VWB DNA were cloned in pBR322, and subsequently mapped. In this manner the restriction map of the VWB phage genome was constructed.
摘要
利用限制性内切酶BglII、ClaI、EcoRI、EcoRV、HindIII、KpnI和SphI构建了肌动噬菌体VWB的物理图谱。噬菌体VWB的基因组大小为47.3 kb，可在委内瑞拉链霉菌上繁殖，也能使其溶源化。VWB DNA经BglII酶切产生的三个片段被克隆到pBR322中，随后进行图谱绘制。通过这种方式构建了VWB噬菌体基因组的限制性图谱。
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