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限制性内切酶Alu I在CHO细胞中的染色体断裂活性与细胞周期的S期无关。

The chromosome-breaking activity of the restriction endonuclease Alu I in CHO cells is independent of the S-phase of the cell cycle.

作者信息

Obe G, Winkel E U

出版信息

Mutat Res. 1985 Oct;152(1):25-9. doi: 10.1016/0027-5107(85)90042-9.

Abstract

The restriction endonuclease Alu I induces chromosomal aberrations in living Chinese hamster ovary (CHO) cells. Multiple fixation times reveal that the chromosome-breaking activity of Alu I is similar to that of ionizing radiation in that it is independent of the S-phase of the cell cycle. These results indicate that DNA double-strand breaks are the ultimate lesions for the production of chromosomal aberrations in all stages of the cell cycle.

摘要

限制性内切酶Alu I可诱导活的中国仓鼠卵巢(CHO)细胞发生染色体畸变。多次固定时间的结果表明,Alu I的染色体断裂活性与电离辐射相似,即它与细胞周期的S期无关。这些结果表明,DNA双链断裂是细胞周期所有阶段产生染色体畸变的最终损伤。

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