从L.叶提取物合成的银纳米颗粒的表征、抗菌、抗氧化和抗凝活性。

Characterization, antimicrobial, antioxidant, and anticoagulant activities of silver nanoparticles synthesized from L. leaf extract.

作者信息

Lateef Agbaje, Folarin Bolaji I, Oladejo Suliat M, Akinola Paul O, Beukes Lorika S, Gueguim-Kana Evariste B

机构信息

a Department of Pure and Applied Biology , Ladoke Akintola University of Technology , Ogbomoso , Nigeria.

b Microscopy and Microanalysis Unit, School of Life Sciences, University of KwaZulu-Natal , Pietermaritzburg , South Africa.

出版信息

Prep Biochem Biotechnol. 2018;48(7):646-652. doi: 10.1080/10826068.2018.1479864. Epub 2018 Jun 29.

Abstract

Phytosynthesis of silver nanoparticles (AgNPs) using leaf extract of () was the focus of this research work. The -AgNPs were characterized by UV-Vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), and selected area electron diffraction (SAED) study. Studies were made on the AgNPs for antibacterial, antifungal, anticoagulant, free-radical scavenging, and hydrogen peroxide scavenging activities. The crystalline -AgNPs were monodispersed, with a size range of 16.70-33.74 nm and maximum absorption at 410 nm. FTIR analysis displayed prominent peaks at 3430.6, 1711.8, and 1165.9/cm, which showed the existence of phenolic compounds and proteins in the synthesis of AgNPs. -AgNPs was active against , , and , with 100% inhibition. The -AgNPs also displayed good antifungal properties, as the concentrations of 100 and 150 µg/mL had 100% inhibition toward and . However, there was 66.67% inhibition of . It scavenged both DPPH and HO by 70.69 and 89.02%, respectively. -AgNPs also prevented the coagulation of human blood. This study, being the first of its kind to use the leaf extract of for the synthesis of AgNPs has shown that -AgNPs can find biomedical applications.

摘要

利用()叶提取物进行银纳米颗粒(AgNPs)的植物合成是本研究工作的重点。通过紫外可见光谱、傅里叶变换红外光谱(FTIR)、透射电子显微镜(TEM)和选区电子衍射(SAED)研究对合成的AgNPs进行了表征。对AgNPs的抗菌、抗真菌、抗凝、自由基清除和过氧化氢清除活性进行了研究。结晶态的合成AgNPs呈单分散状态,尺寸范围为16.70 - 33.74nm,在410nm处有最大吸收。FTIR分析显示在3430.6、1711.8和1165.9/cm处有明显峰值,这表明在AgNPs合成过程中存在酚类化合物和蛋白质。合成的AgNPs对大肠杆菌、金黄色葡萄球菌和枯草芽孢杆菌有活性,抑制率达100%。合成的AgNPs还表现出良好的抗真菌性能,100和150μg/mL浓度对白色念珠菌和热带念珠菌的抑制率达100%。然而,对近平滑念珠菌的抑制率为66.67%。它对DPPH和过氧化氢的清除率分别为70.69%和89.02%。合成的AgNPs还能防止人体血液凝固。本研究首次利用()叶提取物合成AgNPs,结果表明合成的AgNPs可用于生物医学应用。

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索