Geremew Addisie, Carson Laura, Woldesenbet Selamawit
Cooperative Agricultural Research Center, Prairie View A&M University, Prairie View, TX, United States.
Front Mol Biosci. 2022 Sep 20;9:991669. doi: 10.3389/fmolb.2022.991669. eCollection 2022.
The evolution and incidence of multidrug-resistant food-borne pathogens still become a critical public health global issue. To avert this challenge there is great interest in medical applications of silver nanoparticles. Thus, this study aimed to synthesize silver nanoparticles (Rn-AgNPs) using aqueous leaf extract of Nepal Dock ( Spreng) and evaluate their antibacterial potential against food-borne pathogens and antioxidant activity. The Rn-AgNPs were characterized by UV-Vis spectrophotometry, Dynamic Light Scattering (DLS), Scanning Electron Microscopy (SEM), and Fourier Transform Infra-Red Spectroscopy (FTIR). The antibacterial activities of the Rn-AgNPs were evaluated using agar well diffusion (zone of inhibition, ZOI) and microdilution (minimum inhibitory concentration, MIC and minimum bactericidal concentration, MBC) methods. The antioxidant property of the Rn-AgNPs was investigated using radical scavenging (DPPH and hydroxyl) assays. The UV-Vis spectra of Rn-AgNPs elucidated the absorption maxima at 425 nm and FTIR detected numerous functional groups of biological compounds that are responsible for capping and stabilizing Rn-AgNPs. DLS analysis displayed monodispersed Rn-AgNPs of 86.7 nm size and highly negative zeta potential (-32.5 mV). Overall results showed that was the most sensitive organism, whereas was the least sensitive against Rn-AgNPs. In the antioxidant tests, the AgNPs radical scavenging activity reached 95.44% at 100 μg/ml. This study indicates that Rn-AgNPs exhibit a strong antimicrobial on , and and antioxidant and thus might be developed as a new type of antimicrobial agent for the treatment of multidrug-resistant foodborne pathogens and extensible applications in nanomaterial food- and nanocomposite-based antimicrobial packaging and/or as an antioxidant.
多重耐药食源性病原体的演变和发生率仍然是一个关键的全球公共卫生问题。为了应对这一挑战，人们对银纳米颗粒的医学应用产生了浓厚兴趣。因此，本研究旨在利用尼泊尔酸模（Spreng）的水提叶提取物合成银纳米颗粒（Rn-AgNPs），并评估其对食源性病原体的抗菌潜力和抗氧化活性。通过紫外-可见分光光度法、动态光散射（DLS）、扫描电子显微镜（SEM）和傅里叶变换红外光谱（FTIR）对Rn-AgNPs进行了表征。采用琼脂孔扩散法（抑菌圈，ZOI）和微量稀释法（最低抑菌浓度，MIC和最低杀菌浓度，MBC）评估了Rn-AgNPs的抗菌活性。采用自由基清除（DPPH和羟基）试验研究了Rn-AgNPs的抗氧化性能。Rn-AgNPs的紫外-可见光谱显示在425nm处有最大吸收峰，FTIR检测到许多负责包覆和稳定Rn-AgNPs的生物化合物官能团。DLS分析显示Rn-AgNPs单分散，尺寸为86.7nm，zeta电位高度为负（-32.5mV）。总体结果表明，[具体菌株1]是对Rn-AgNPs最敏感的菌株，而[具体菌株2]对Rn-AgNPs最不敏感。在抗氧化试验中，AgNPs在100μg/ml时的自由基清除活性达到95.44%。本研究表明，Rn-AgNPs对[具体菌株1]、[具体菌株2]和[具体菌株3]具有较强的抗菌作用以及抗氧化作用，因此可能被开发为一种新型抗菌剂，用于治疗多重耐药食源性病原体，并可广泛应用于基于纳米材料的食品和纳米复合材料抗菌包装中，和/或作为抗氧化剂。
