Isberg R R, Falkow S
Nature. 1985;317(6034):262-4. doi: 10.1038/317262a0.
For many species of pathogenic bacteria, invasion and survival within animal cells is central to establishing a successful host-parasite relationship. Localization within host cells protects the microorganism from host defences, or permits it to cross epithelial barriers and subsequently become systemically distributed. The precise mechanisms that permit entry of bacteria into host tissues are unclear, therefore we have been studying the invasion of epithelial cells by Yersinia pseudotuberculosis. As a first step towards identifying the factors required for this process, we report here the identification of a single genetic locus from this organism that is sufficient to convert the innocuous Escherichia coli K-12 strain into an organism capable of invading cultured animal cells.
对于许多病原菌而言，在动物细胞内的侵袭和存活是建立成功的宿主-寄生虫关系的核心。定位于宿主细胞内可保护微生物免受宿主防御机制的影响，或使其能够穿过上皮屏障并随后进行全身分布。细菌进入宿主组织的精确机制尚不清楚，因此我们一直在研究假结核耶尔森菌对上皮细胞的侵袭。作为确定该过程所需因素的第一步，我们在此报告从该生物体中鉴定出一个单一的基因位点，该位点足以将无害的大肠杆菌K-12菌株转化为能够侵袭培养的动物细胞的生物体。
