Guo Huanping, Yin Chunsheng, Galon Eloiza May, Du Jige, Gao Yang, Adjou Moumouni Paul Franck, Liu Mingming, Efstratiou Artemis, Lee Seung-Hun, Li Jixu, Ringo Aaron Edmond, Wang Guanbo, Li Yongchang, Tumwebaze Maria Agnes, Xuan Xuenan
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan.
China Institute of Veterinary Drug Control, Beijing 100081, China.
Parasitol Int. 2018 Dec;67(6):679-683. doi: 10.1016/j.parint.2018.06.011. Epub 2018 Jun 27.
Theileriosis and ehrlichiosis are two important tick-borne diseases affecting cattle farming in China. However, limited information is available regarding prevalence and molecular characterization of Theileria annulata and Ehrlichia ruminantium in cattle in Xinjiang Uygur Autonomous Region (XUAR), northwestern China. In this study, a total of 176 blood samples of cattle from three rural areas of XUAR were collected in June 2017 and were tested by nested-PCR. A total of 34 (19.3%) samples were found to be infected with one or two pathogens. The overall prevalence rates of T. annulata and E. ruminantium were 18.2% and 1.7%, respectively. Phylogenetic analyses revealed that the E. ruminantium isolates from XUAR were located in the same clade but diverged from the isolates from African countries using pCS20 gene while T. annulata isolates from XUAR revealed differences in the genotypes using Tams1 sequences. To our knowledge, this is the first report of E. ruminantium infection in cattle in China. It also provides the first genetic characterization of T. annulata in cattle in XUAR. The current findings are important for understanding the distribution of agents of theileriosis and ehrlichiosis and in designing measures for the prevention and control of tick-borne diseases in cattle, other animals, and humans.
泰勒虫病和埃立克体病是影响中国养牛业的两种重要蜱传疾病。然而，关于中国西北部新疆维吾尔自治区（XUAR）牛体内环形泰勒虫和反刍动物埃立克体的流行情况和分子特征的信息有限。在本研究中，2017年6月从XUAR三个农村地区共采集了176份牛血样，并通过巢式PCR进行检测。共发现34份（19.3%）样本感染了一种或两种病原体。环形泰勒虫和反刍动物埃立克体的总体流行率分别为18.2%和1.7%。系统发育分析表明，利用pCS20基因，来自XUAR的反刍动物埃立克体分离株位于同一进化枝，但与来自非洲国家的分离株不同，而利用Tams1序列，来自XUAR的环形泰勒虫分离株在基因型上存在差异。据我们所知，这是中国牛感染反刍动物埃立克体的首次报道。它还提供了XUAR牛体内环形泰勒虫的首次基因特征。目前的研究结果对于了解泰勒虫病和埃立克体病病原体的分布以及制定牛、其他动物和人类蜱传疾病的预防和控制措施具有重要意义。
