Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.
Chair and Department of Cardiology, Hypertension and Internal Medicine, Second Faculty of Medicine, Medical University of Warsaw, Mazovian Bródnowski Hospital, Warsaw, Poland.
Sci Rep. 2018 Jun 29;8(1):9883. doi: 10.1038/s41598-018-28118-1.
Left ventricular (LV) dysfunction after acute myocardial infarction (AMI) is associated with an increased risk of heart failure (HF) development. Diverse microRNAs (miRNAs) have been shown to appear in the bloodstream following various cardiovascular events. The aim of this study was to identify prognostic miRNAs associated with LV dysfunction following AMI. Patients were divided into subgroups comprising patients who developed or not LV dysfunction within six months of the infarction. miRNA profiles were determined in plasma and serum samples of the patients on the first day of AMI. Levels of 14 plasma miRNAs and 16 serum miRNAs were significantly different in samples from AMI patients who later developed LV dysfunction compared to those who did not. Two miRNAs were up-regulated in both types of material. Validation in an independent group of patients, using droplet digital PCR (ddPCR) confirmed that miR-30a-5p was significantly elevated on admission in those patients who developed LV dysfunction and HF symptoms six months after AMI. A bioinformatics analysis indicated that miR-30a-5p may regulate genes involved in cardiovascular pathogenesis. This study demonstrates, for the first time, a prognostic value of circulating miR-30a-5p and its association with LV dysfunction and symptoms of HF after AMI.
急性心肌梗死后左心室（LV）功能障碍与心力衰竭（HF）发展风险增加相关。多种 microRNAs（miRNAs）已被证明在各种心血管事件后出现在血液中。本研究旨在确定与 AMI 后 LV 功能障碍相关的预后 miRNAs。患者分为亚组，包括在梗死后 6 个月内发生或未发生 LV 功能障碍的患者。在 AMI 患者发病的第一天，用血浆和血清样本测定 miRNA 图谱。与未发生 LV 功能障碍的患者相比，发生 LV 功能障碍的 AMI 患者的血浆和血清样本中，14 种血浆 miRNA 和 16 种血清 miRNA 的水平有显著差异。两种 miRNA 在两种类型的材料中均上调。在另一组患者中使用液滴数字 PCR（ddPCR）进行验证，证实 miR-30a-5p 在 AMI 后 6 个月发生 LV 功能障碍和 HF 症状的患者入院时明显升高。生物信息学分析表明，miR-30a-5p 可能调节与心血管发病机制相关的基因。这项研究首次证明了循环 miR-30a-5p 的预后价值及其与 AMI 后 LV 功能障碍和 HF 症状的关系。
