Zheng Dahai, Chen Jianzhu
Interdisciplinary Research Group in Infectious Diseases, Singapore-Massachusetts Institute of Technology Alliance for Research and Technology, Singapore, Singapore.
The Koch Institute for Integrative Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA.
Methods Mol Biol. 2018;1817:33-40. doi: 10.1007/978-1-4939-8600-2_4.
Club cells are a major bronchiolar epithelial cell type in the lung. Using genetic lineage tracing in mice and in vitro culture of purified cells, we have shown that club cells can differentiate into alveolar type I and II cells. Here we describe the detailed protocol for culturing and differentiating club cells in 3-dimensional culture.
俱乐部细胞是肺中主要的细支气管上皮细胞类型。通过在小鼠中进行遗传谱系追踪以及对纯化细胞进行体外培养，我们已经表明俱乐部细胞可以分化为I型和II型肺泡细胞。在此，我们描述了在三维培养中培养和分化俱乐部细胞的详细方案。
