Institute of Life Sciences, MOE Key Laboratory of Developmental Genes and Human Diseases, Southeast University, Nanjing, 210096, China; Southeast University Medical School, Nanjing, 210009, China.
Institute of Life Sciences, MOE Key Laboratory of Developmental Genes and Human Diseases, Southeast University, Nanjing, 210096, China.
Biochem Biophys Res Commun. 2018 Sep 5;503(2):1148-1153. doi: 10.1016/j.bbrc.2018.06.133. Epub 2018 Jul 4.
Magnesium transporter subtype 1 (MagT1) is a magnesium membrane transporter with channel like properties. We have previously identified MagT1 (CG7830) in Drosophila genome and characterized its protein product by electrophysiological means. Here, we report the generation of fly MagT1 mutants and show that MagT1 is essential for early embryonic development. In wings and primordial wings, by clonal analysis and RNAi knock down of MagT1, we have found that loss of MagT1 results in enhanced/ectopic Wingless (Wg, a fly Wnt) signaling and disrupted Decapentaplegic (Dpp) signaling, indicating the crucial role of MagT1 for fly development at later stages. Finally, we demonstrate directly that magnesium transportations are proportional with the MagT1 expressional levels in Drosophila S2 cells. Taken together, these findings may suggest that MagT1 is a major magnesium transporter/channel profoundly involved in fly development by affecting developmental signaling pathways, such as Wg and Dpp signaling.
镁转运蛋白亚型 1（MagT1）是一种具有通道样特性的镁膜转运蛋白。我们之前在果蝇基因组中鉴定出 MagT1（CG7830），并通过电生理学方法对其蛋白质产物进行了表征。在这里，我们报告了果蝇 MagT1 突变体的产生，并表明 MagT1 对早期胚胎发育是必需的。在翅膀和原基翅膀中，通过克隆分析和 MagT1 的 RNAi 敲低，我们发现 MagT1 的缺失导致 Wingless（Wg，一种果蝇 Wnt）信号的增强/异位和 Decapentaplegic（Dpp）信号的破坏，表明 MagT1 在后期对果蝇发育起着至关重要的作用。最后，我们直接证明了在果蝇 S2 细胞中，镁的转运与 MagT1 的表达水平成正比。总之，这些发现可能表明 MagT1 是一种主要的镁转运蛋白/通道，通过影响发育信号通路，如 Wg 和 Dpp 信号通路，在果蝇发育中起着重要作用。
