Qin Chuan, Guo Yun, Yang De-Gang, Yang Ming-Liang, Du Liang-Jie, Li Jian-Jun
Department of Spinal and Neural Functional Reconstruction, China Rehabilitation Research Center, Beijing Boai Hospital, Beijing, China.
School of Rehabilitation Medicine, Capital Medical University, Beijing, China.
Cell Physiol Biochem. 2018;47(5):1835-1852. doi: 10.1159/000491064. Epub 2018 Jun 29.
BACKGROUND/AIMS: Spinal cord injury (SCI) has long been a subject of great interest in a wide range of scientific fields. Several attempts have been made to demonstrate motor function improvement in rats with SCI after transplantation of induced pluripotent stem cells (iPSC). This systematic review and meta-analysis was designed to summarize the effects of iPSC on locomotor recovery in rat models of SCI.
We searched the publications in the PubMed, Medline, Science Citation Index, Cochrane Library, CNKI, and Wan-fang databases and the China Biology Medicine disc. Results were analyzed by Review Manager 5.3.0. The quality of evidence was assessed using the Grading of Recommendations Assessment, Development and Evaluation (GRADE) methodology.
Six randomized controlled preclinical trials covering eight comparisons and including 212 rats were selected. The subgroup analyses were based on the following items: different SCI models, cell counts, iPSC sources, iPSC differentiations and transplantation methods. The pooled results indicated that iPSC transplantation significantly improved locomotor recovery of rats after SCI by sustaining beneficial effects, especially in the subgroups of contusion, moderate cell counts (5×105), source of human fetal lung fibroblasts, iPSC-neural precursors and intraspinal injection.
Our meta-analysis of the effects of iPSC transplantation on locomotor function in SCI models is, to our knowledge, the first meta-analysis in this field. We conclude that iPSC transplantation improves locomotor recovery in rats with SCI, implicating this strategy as an effective therapy. However, more studies are required to validate our conclusions.
背景/目的：脊髓损伤（SCI）长期以来一直是众多科学领域中备受关注的课题。人们已经进行了多项尝试，以证明在诱导多能干细胞（iPSC）移植后，脊髓损伤大鼠的运动功能有所改善。本系统评价和荟萃分析旨在总结iPSC对脊髓损伤大鼠模型运动恢复的影响。
我们检索了PubMed、Medline、科学引文索引、Cochrane图书馆、中国知网、万方数据库以及中国生物医学光盘中的出版物。使用Review Manager 5.3.0对结果进行分析。采用推荐分级评估、制定和评价（GRADE）方法评估证据质量。
选取了6项随机对照临床前试验，涵盖8项比较，包括212只大鼠。亚组分析基于以下项目：不同的脊髓损伤模型、细胞计数、iPSC来源、iPSC分化和移植方法。汇总结果表明，iPSC移植通过维持有益作用显著改善了脊髓损伤大鼠的运动恢复，特别是在挫伤、中等细胞计数（5×105）、人胎儿肺成纤维细胞来源、iPSC神经前体细胞和脊髓内注射的亚组中。
据我们所知，我们对iPSC移植对脊髓损伤模型运动功能影响的荟萃分析是该领域的首次荟萃分析。我们得出结论，iPSC移植可改善脊髓损伤大鼠的运动恢复，表明该策略是一种有效的治疗方法。然而，需要更多的研究来验证我们的结论。
