Qiang Hong, Li Yu-You, Pei Meng-Fu
College of Natural Resources and Environment, Northwest A & F University, Yangling 712100, China.
Department of Civil and Environmental Engineering, Graduate School of Engineering, Tohoku University, Sendai 980-8579, Japan.
Huan Jing Ke Xue. 2018 Jul 8;39(7):3443-3451. doi: 10.13227/j.hjkx.201709199.
In order to assess the sulfate-induced inhibition of anaerobic digestion of antibiotic manufacturing bio-waste. The effect of COD/SO ratio on biogas production potential and substrate utilization characteristics during the acidogenic phase of anaerobic digestion of penicillin bacterial residues were investigated through batch experiments. The results obtained indicated that biogas production was gradually enhanced after 10 days of anaerobic digestion. However, the maximum cumulative methane production probably exceeded about 208 mL·g(on TS basis) since COD/SO≥3. Because adaptive acclimation, more than 71% COD removal, which may have been converted to methane, was achieved. Consequently, at COD/SO≤1.5, methane production was suppressed by 49% and 100% when the organics and SO removal rates were less than 17% and 5%, respectively. This indicated that methanogens and sulfate-reducing bacteria were inhibited at high sulfate loading rates. In addition, the COD balance analysis revealed that less than 9.1% of the COD was converted to methane. However, 5.0%-9.0% of the COD was used for sulfate reduction. This means that methanogens are more susceptible than sulfate-reducing bacteria to sulfide-induced inhibition. The S balance showed that the reduced sulfate was mainly present as sulfide in the digester. A small fraction of it was present as hydrogen sulfide in the biogas. The analysis of substrate utilization characteristics during the acidogenic phase revealed that methanation of soluble protein was initiated after methanation of soluble carbohydrate.
为了评估硫酸盐对抗生素生产生物废弃物厌氧消化的抑制作用。通过批次实验研究了COD/SO比在青霉素菌渣厌氧消化产酸阶段对沼气生产潜力和底物利用特性的影响。所得结果表明，厌氧消化10天后沼气产量逐渐增加。然而，当COD/SO≥3时，最大累积甲烷产量可能超过约208 mL·g（基于TS）。由于适应性驯化，实现了超过71%的COD去除，这些COD可能已转化为甲烷。因此，当COD/SO≤1.5，且有机物和SO去除率分别低于17%和5%时，甲烷产量分别被抑制了49%和100%。这表明在高硫酸盐负荷率下，产甲烷菌和硫酸盐还原菌受到抑制。此外，COD平衡分析表明，不到9.1%的COD转化为甲烷。然而，5.0%-9.0%的COD用于硫酸盐还原。这意味着产甲烷菌比硫酸盐还原菌更容易受到硫化物诱导的抑制。S平衡表明，还原的硫酸盐主要以硫化物的形式存在于消化池中。其中一小部分以硫化氢的形式存在于沼气中。对产酸阶段底物利用特性的分析表明，可溶性蛋白质的甲烷化在可溶性碳水化合物甲烷化之后开始。
