Liu Wei, Zhao Donghai, Wang Ziyan, Li Yan, Wang Hao, Wang Huiyan
Bing Du Xue Bao. 2016 May;32(3):267-72.
Herpes simplex virus 2 (HSV-2) is the main pathogen of genital herpes (GH).The currently available body of evidence suggests that only widespread use of an effective vaccine can control the spread of HSV-2.We developed a recombinant replication-defective adenovirus capable of expressing glycoprotein D2(rAd-gD2), and was identified by PCR and Western blot in gene and protein level, respectively. The mice in test groups were immunized intramuscularly (i.m.) with rAd-gD2 in 0and 4 week,while control mice received PBS and AdV. We detected gD2 IgG antibody and neutralizing antibody in order to evaluate of humoral immune response, detected Th1 (IL-2 and IFN-γ)/Th2 (IL-4, IL-5 and IL-10) cytokines in order to evaluate of cellular immune response. The results showed that immunization with rAd-gD2 could elicit strong gD2IgG(P<0.01)and neutralizing antibody levels(P<0.05),which were significantly higher than that with PBS and AdV groups IL-2IFN-γIL-4IL-5 and IL-10 detected in rAd-gD2 group were higher than control groups (P<0.01), Th1/Th2 cytokines were important standard of cellular/humoral immune response, respectively. The results demonstrated that rAd-gD2 could stimulate not only humoral immune response but also cellular immune response, rAd-gD2 may be good candidate for the control of HSV-2infection.
单纯疱疹病毒2型（HSV-2）是生殖器疱疹（GH）的主要病原体。目前可得的证据表明，只有广泛使用有效的疫苗才能控制HSV-2的传播。我们构建了一种能够表达糖蛋白D2的重组复制缺陷型腺病毒（rAd-gD2），并分别通过PCR和蛋白质印迹在基因和蛋白质水平进行了鉴定。实验组小鼠在第0周和第4周通过肌肉注射（i.m.）rAd-gD2进行免疫，而对照小鼠接受PBS和腺病毒载体（AdV）。我们检测了gD2 IgG抗体和中和抗体以评估体液免疫反应，检测了Th1（IL-2和IFN-γ）/Th2（IL-4、IL-5和IL-10）细胞因子以评估细胞免疫反应。结果表明，用rAd-gD2免疫可引发强烈的gD2 IgG（P<0.01）和中和抗体水平（P<0.05），显著高于PBS和AdV组。在rAd-gD2组中检测到的IL-2、IFN-γ、IL-4、IL-5和IL-10高于对照组（P<0.01），Th1/Th2细胞因子分别是细胞/体液免疫反应的重要指标。结果表明，rAd-gD2不仅能刺激体液免疫反应，还能刺激细胞免疫反应，rAd-gD2可能是控制HSV-2感染的良好候选物。
