Bairami Amir, Rezaei Sasan, Rezaeian Mostafa
Dept. of Medical Parasitology and Mycology, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran.
Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Iran J Parasitol. 2018 Jan-Mar;13(1):24-30.
Diarrheal disease annually causes 760000 deaths in children, and 1700 million new cases are reported each year worldwide. Among the parasites, , , and spp. are the most important infectious agents leading to diarrhea. Clinical presentations due to these parasites are more or less similar, and microscopy is not as much as sensitive for the detection. The aim of this study was to set up and evaluate a Multiplex PCR Assay for Synchronous Identification of , , and spp. in Stool Samples.
Samples were obtained from different sources such as culture media and patient stool samples. Primer pairs were designed using primer-BLAST, and for the extraction of DNA, the QIAamp DNA stool mini kit was used. The study was conducted in Tehran, Iran and completed in 2016.
The current multiplex PCR assay for the detection of achieved sensitivity and specificity of 86.36% (95% CI: 65.09% to 97.09) and 95.74 % (95% CI: 85.46% to 99.48%), respectively. Sensitivity and specificity of the test for was 90.91% (95% CI: 70.84% to 98.88%) and 95.74% (95%CI: 85.46% to 99.48%), respectively, and for the detection of , multiplex PCR showed a sensitivity of 90.91% (95% CI: 70.84% to 98.88%) and specificity of 95.74% (95%CI: 85.46% to 99.48%).
Multiplex PCR in this study showed admissible sensitivity and specificity for the detection of , , and spp. in fecal samples.
腹泻病每年导致76万儿童死亡，全球每年报告17亿新病例。在寄生虫中， 、 和 属是导致腹泻的最重要感染因子。这些寄生虫引起的临床表现或多或少相似，显微镜检查对其检测的敏感性不高。本研究的目的是建立并评估一种用于同步鉴定粪便样本中 、 和 属的多重聚合酶链反应（PCR）检测方法。
样本取自不同来源，如培养基和患者粪便样本。使用引物设计软件Primer-BLAST设计引物对，采用QIAamp DNA粪便微量提取试剂盒提取DNA。该研究在伊朗德黑兰进行，于2016年完成。
当前用于检测 的多重PCR检测方法的灵敏度和特异性分别为86.36%（95%置信区间：65.09%至97.09）和95.74%（95%置信区间：85.46%至99.48%）。检测 的试验灵敏度和特异性分别为90.91%（95%置信区间：70.84%至98.88%）和95.74%（95%置信区间：85.46%至99.48%），而对于 的检测，多重PCR显示灵敏度为90.91%（95%置信区间：70.84%至98.88%），特异性为95.74%（95%置信区间：85.46%至99.48%）。
本研究中的多重PCR在检测粪便样本中的 、 和 属时显示出可接受的灵敏度和特异性。
