Ambroziak Katarzyna, Adamowicz Piotr
Institute of Forensic Research, Westerplatte 9, 31-033 Kraków, Poland.
Forensic Toxicol. 2018;36(2):280-290. doi: 10.1007/s11419-017-0401-x. Epub 2018 Jan 31.
In recent years, many synthetic cannabinoids (SCs) have appeared on the drug market. Despite the increasing number of SCs, there are few comprehensive screening methods for their detection in biological specimens. In this context, the purpose of this study was to develop a fast and simple liquid chromatography-tandem mass spectrometry screening procedure for detection and identification of SCs in whole blood.
The elaborated qualitative screening method allows the simultaneous detection and identification of 72 compounds from different chemical groups: naphthoylindoles, naphthoylindazoles, benzoylindoles, phenylacetylindoles, tetramethylcyclopropylindoles, indole-3-carboxylic acid esters, indole-3-carboxylic acid amides, indazole-3-carboxylic acid amides, and others. Whole-blood samples (0.2 mL) were precipitated with acetonitrile (0.6 mL). The separation was achieved with the gradient of the mobile phase composition (0.1% formic acid in acetonitrile and 0.1% formic acid in water) and the gradient of the flow rate (0.5-0.8 mL/min) in 16 min. Detection of all compounds was based on dynamic multiple reaction monitoring.
Mass spectrometer parameters for all compounds were presented. All of the compounds were well-separated by their retention times and/or transitions. The limits of detection (LODs) for 50 compounds were in the range 0.01-0.48 ng/mL.
Estimated LODs make this assay suitable for the analysis of biological material. The procedure can be easily expanded for more substances, which is an indispensable advantage in the dynamically developing drug market. It can have wide application in various analytical forensic and clinical laboratories.
近年来，许多合成大麻素（SCs）出现在毒品市场上。尽管合成大麻素的数量不断增加，但在生物样本中检测它们的综合筛查方法却很少。在此背景下，本研究的目的是开发一种快速简便的液相色谱 - 串联质谱筛查程序，用于检测和鉴定全血中的合成大麻素。
精心制定的定性筛查方法可同时检测和鉴定来自不同化学组的72种化合物：萘甲酰吲哚、萘甲酰吲唑、苯甲酰吲哚、苯乙酰吲哚、四甲基环丙基吲哚、吲哚 - 3 - 羧酸酯、吲哚 - 3 - 羧酸酰胺、吲唑 - 3 - 羧酸酰胺等。全血样本（0.2 mL）用乙腈（0.6 mL）沉淀。通过流动相组成（乙腈中0.1%甲酸和水中0.1%甲酸）的梯度和流速（0.5 - 0.8 mL/min）的梯度在16分钟内实现分离。所有化合物的检测基于动态多反应监测。
给出了所有化合物的质谱仪参数。所有化合物通过保留时间和/或跃迁得到了很好的分离。50种化合物的检测限（LOD）在0.01 - 0.48 ng/mL范围内。
估计的检测限使该测定法适用于生物材料的分析。该程序可以很容易地扩展用于更多物质，这在动态发展的毒品市场中是一个不可或缺的优势。它可在各种法医和临床分析实验室中广泛应用。
