Hess Cornelius, Murach Jacqueline, Krueger Lynn, Scharrenbroch Lisa, Unger Michael, Madea Burkhard, Sydow Konrad
Institute of Forensic Medicine, University Bonn, Department Forensic Toxicology, Stiftsplatz 12, 53111, Bonn, Germany.
Drug Test Anal. 2017 May;9(5):721-733. doi: 10.1002/dta.2030. Epub 2016 Jul 12.
The Internet is flooded with steadily changing synthetic cannabinoids in Spice products. In routine forensic work, it is difficult to keep the analytical methods for the detection of these analytes up to date. We describe a liquid chromatography-tandem mass spectrometry method after liquid-liquid extraction for the detection of 93 synthetic cannabinoids in human serum. The method was validated for selectivity and specificity, matrix effects, and analytical limits (<1 ng/mL for 81 substances) for qualitative analysis. A short quantitative validation regarding linearity and precision data was also conducted. The method was applied to 189 serum samples provided by police authorities. Sixty-four samples (33.8%) were found positive for at least one synthetic cannabinoid, whereby MDMB-CHMICA, AB-CHMINACA, and 5 F-PB-22 were the substances most frequently detected. Consumption of these substances and plasma concentrations are linked to symptoms documented by the police. Six case reports are presented. Copyright © 2016 John Wiley & Sons, Ltd.
互联网上充斥着“香料”产品中不断变化的合成大麻素。在常规法医工作中，很难使检测这些分析物的分析方法与时俱进。我们描述了一种液液萃取后采用液相色谱 - 串联质谱法检测人血清中93种合成大麻素的方法。该方法针对定性分析的选择性和特异性、基质效应及分析限（81种物质<1 ng/mL）进行了验证。还进行了关于线性和精密度数据的简短定量验证。该方法应用于警方提供的189份血清样本。64份样本（33.8%）被检测出至少含有一种合成大麻素呈阳性，其中MDMB-CHMICA、AB-CHMINACA和5F-PB-22是最常检测到的物质。这些物质的消费情况和血浆浓度与警方记录的症状相关。本文还呈现了6例病例报告。版权所有© 2016约翰威立父子有限公司。
