Anderson J M, Keefer D A, Koritnik D D, Yasumoto T, Cronin M J
Am J Physiol. 1985 Oct;249(4 Pt 1):E337-44. doi: 10.1152/ajpendo.1985.249.4.E337.
We investigated the effects of dopamine, forskolin, and maitotoxin on prolactin release from individual rat and monkey mammotrophs. Forskolin increases cAMP levels, whereas maitotoxin amplifies the influx of extracellular calcium. Prolactin secretion from single mammotrophs was visualized by the reverse hemolytic plaque assay and then quantified by measuring the proportion of plaque-forming cells and the mean plaque area. In the presence of dopamine alone both the plaque proportion and mean area of the plaques formed by rat mammotrophs decreased by 50 and 40%, respectively. This inhibition of secretion was blocked by the dopaminergic antagonist spiperone as well as forskolin and maitotoxin. Forskolin and maitotoxin alone significantly elevated both the proportion (+30%) and area (+180% for forskolin and +250% for maitotoxin) of the plaques. These actions of maitotoxin were neutralized by D-600, a calcium channel blocker. All of these agents induced similar trends with monkey prolactin cells. We conclude that single mammotrophs in culture respond to perturbations in a differential manner and in a way predicted by earlier results based on macropopulation measurements.
我们研究了多巴胺、福斯高林和 maitotoxin 对单个大鼠和猴乳腺营养细胞催乳素释放的影响。福斯高林可提高环磷酸腺苷（cAMP）水平，而 maitotoxin 会增加细胞外钙的内流。通过反向溶血空斑试验观察单个乳腺营养细胞的催乳素分泌情况，然后通过测量空斑形成细胞的比例和平均空斑面积进行定量分析。仅在多巴胺存在的情况下，大鼠乳腺营养细胞形成的空斑比例和平均面积分别下降了50%和40%。多巴胺能拮抗剂螺哌隆以及福斯高林和 maitotoxin 可阻断这种分泌抑制作用。单独使用福斯高林和 maitotoxin 可使空斑比例（分别增加30%）和面积（福斯高林增加180%，maitotoxin 增加250%）显著升高。maitotoxin 的这些作用可被钙通道阻滞剂 D - 600 中和。所有这些药物对猴催乳素细胞都产生了类似的趋势。我们得出结论，培养中的单个乳腺营养细胞对干扰的反应具有差异性，且其反应方式与早期基于大群体测量结果所预测的方式一致。
