Key Laboratory of Animal Physiology and Biochemistry, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, PR China; School of Food Science, Nanjing Xiaozhuang University, Nanjing 211171, PR China; Jiangsu Provincial Key Construction Laboratory of Special Biomass Byproduct Resource Utilization, 211171, PR China.
School of Food Science, Nanjing Xiaozhuang University, Nanjing 211171, PR China; Jiangsu Provincial Key Construction Laboratory of Special Biomass Byproduct Resource Utilization, 211171, PR China.
Int J Biol Macromol. 2018 Oct 15;118(Pt A):886-895. doi: 10.1016/j.ijbiomac.2018.06.140. Epub 2018 Jun 28.
Chicory polysaccharides (CP) from Cichorium intybus L. roots were extracted and fractionated to isolate two novel polysaccharide fractions, CP-1 and CP-2. CP-1 is a heteropolysaccharide that is mainly composed of sorbin, glucose, fructose, and glucitol at a molar ratio of 1.00:5.58:13.97:10.32. The molecular weight of CP-1 was 8511.4 Da. The hepatoprotective effect of CP-1 was investigated in a rat model of high-fat diet induced non-alcoholic fatty liver disease (NAFLD) rats. Results indicated that the oral administration of CP-1 significantly decreased body weight and liver index in NAFLD rats. CP-1 also significantly increased serum levels of SOD and HDLC, and decreased the levels of ALT, AST, TG, TC, LDL-C, GLU, ALP, LDH, and MDA in NAFLD rats. Meanwhile, CP-1 effectively decreased MDA, TC, and TG, and increased SOD and T-AOC in the livers of NAFLD rats. Furthermore, CP-1 also increased the hepatic expression of p-AMPKα, ATGL, CPT-1, and p-ACC, and reduced the hepatic expression of ACC, FAS, and SCD-1. Moreover, histopathological examination of the livers showed that CP-1 significantly ameliorated the symptoms of NAFLD rats. Therefore, CP-1 significantly attenuated the high-fat diet-induced NAFLD in rats via AMPK activation.
菊苣多糖（CP）从菊苣根中提取并分离得到两种新型多糖组分 CP-1 和 CP-2。CP-1 是一种杂多糖，主要由山梨醇、葡萄糖、果糖和肌醇以摩尔比 1.00:5.58:13.97:10.32 组成。CP-1 的分子量为 8511.4 Da。研究了 CP-1 在高脂饮食诱导的非酒精性脂肪性肝病（NAFLD）大鼠模型中的保肝作用。结果表明，CP-1 口服给药可显著降低 NAFLD 大鼠的体重和肝指数。CP-1 还可显著提高血清 SOD 和 HDLC 水平，降低 ALT、AST、TG、TC、LDL-C、GLU、ALP、LDH 和 MDA 在 NAFLD 大鼠中的水平。同时，CP-1 可有效降低 MDA、TC 和 TG，并增加 SOD 和 T-AOC 在 NAFLD 大鼠肝脏中的含量。此外，CP-1 还可增加肝脏中 p-AMPKα、ATGL、CPT-1 和 p-ACC 的表达，并降低 ACC、FAS 和 SCD-1 的表达。此外，肝脏组织病理学检查显示 CP-1 可显著改善 NAFLD 大鼠的症状。因此，CP-1 通过激活 AMPK 显著减轻了高脂肪饮食诱导的大鼠 NAFLD。
