Proteomics & Molecular Cell Physiology Laboratory, School of Life Sciences, Bharathiar University, Coimbatore, 641 046, TN, India.
Proteomics & Molecular Cell Physiology Laboratory, School of Life Sciences, Bharathiar University, Coimbatore, 641 046, TN, India.
Photodiagnosis Photodyn Ther. 2018 Sep;23:244-253. doi: 10.1016/j.pdpdt.2018.06.025. Epub 2018 Jun 28.
Herein, we posit a biocompatible and pH-switchable integrated nano-delivery of CBP/ICG to the in vitro and in vivo experiments demonstrate that nanoparticles (NPs) have insignificant toxicity and good biocompatibility, and possess excellent tumor targeting efficiency as evidenced by highly efficient tumor ablation under near -infrared (NIR) illumination. In addition, we have conjugated folic acid as a targeting ligand for folate receptor-targeted delivery. Particularly, targeted delivery of dual CBP/ICG loaded NPs provide targeted detection and transporting potential to specific receptor-expressing tumors, and then CBP interfering with DNA damage and ICG generates singlet oxygen as well as photothermal heat when irradiated with NIR for simultaneous trimodal PDT/PTT/Chemotherapy. Using an animal model, a dramatic reduction in tumor growth without any evidence of significant long-term toxicity to organs after administration of NPs for trimodal therapy subjecting to NIR illumination. Thus, the in vivo satisfactory antitumor trimodal combined efficacy concurrent with complete tumor eradication and promising potential for advanced clinical phototherapy.
在这里，我们提出了一种生物相容且 pH 可切换的 CBP/ICG 集成纳米递药系统。体外和体内实验证明，纳米粒子（NPs）具有较小的毒性和良好的生物相容性，并具有优异的肿瘤靶向效率，在近红外（NIR）照射下可实现高效的肿瘤消融。此外，我们还将叶酸偶联作为叶酸受体靶向递药的靶向配体。特别是，双重 CBP/ICG 负载 NPs 的靶向递药为特定受体表达肿瘤提供了靶向检测和输送的潜力，然后 CBP 干扰 DNA 损伤，ICG 在近红外光照射下产生单线态氧和光热，从而实现光动力治疗/光热治疗/化学治疗的三联治疗。在动物模型中，在给予 NPs 进行三联治疗并接受近红外光照射后，肿瘤生长明显减少，而对器官没有明显的长期毒性证据。因此，体内令人满意的抗肿瘤三联联合疗效，同时完全消除肿瘤，并具有先进临床光疗的广阔应用前景。
