Departamento de Química, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Ruta Nacional 36 Km 601, X5804BYA Río Cuarto, Córdoba, Argentina.
Departamento de Química, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Ruta Nacional 36 Km 601, X5804BYA Río Cuarto, Córdoba, Argentina.
Photodiagnosis Photodyn Ther. 2018 Sep;23:261-269. doi: 10.1016/j.pdpdt.2018.06.020. Epub 2018 Jun 30.
Silica nanoparticles (SiNPs) embedded with Zn(II) 2,9,16,23-tetrakis(methoxy)phthalocyanine (SiNPZnPcOCH), Zn(II) 2,9,16,23-tetrakis(4-pyridyloxy) phthalocyanine (SiNPZnPcOPy) and Zn(II) 2,9,16,23-tetrakis(t-butyl) phthalocyanine (SiNPZnPctBu) were synthesized in the nonpolar core of AOT/1-butanol/water micelles using triethoxyvinylsilane and 3-aminopropyltriethoxysilane. These SiNPs-Pc presented an average diameter of about 20-25 nm. UV-vis absorption spectra presented the characteristic Soret and Q bands of phthalocyanines embedded into the nanoparticles. Moreover, red fluorescence emission of SiNPs bearing phthalocyanines was detected in water. The SiNPs-Pc produced the photodecomposition of 2,2'-(anthracene-9,10-diyl)bis(methylmalonic acid), which was used to sense the singlet molecular oxygen O(Δ) generation in aqueous medium. Also, the formation of superoxide anion radical was detected by nitro blue tetrazolium reduction in the presence of NADH. Photoinactivation of microorganisms was investigated in Staphylococcus aureus and Candida albicans. In vitro experiments showed that photosensitized inactivation induced by SiNPZnPcOCH and SiNPZnPctBu improved with an increase of irradiation times. After 30 min irradiation, over 7 log reduction was found for S. aureus. Also, these SiNPs-Pc produced a decrease of 2.5 log in C. albicans after 60 min irradiation. In both cases, a lower photoinactivation activity was found for SiNPZnPcOPy. Studies of photodynamic action mechanism showed that the photokilling of microbial cells was protected in the presence of sodium azide and diazabicyclo[2.2.2]octane. Also, a reduction on the cell photodamage was found with the addition of D-mannitol. Therefore, the photodynamic activity sensitized by SiNPZnPcOCH and SiNPZnPctBu in microbial cells was mediated by a contribution of both type I and type II photooxidative mechanisms. Thus, silica nanoparticles are interesting materials to vehicle ZnPcOCH and ZnPctBu in aqueous media to photoeradicate microorganisms.
硅纳米粒子（SiNPs）嵌入了 Zn(II) 2,9,16,23-四（甲氧基）酞菁（SiNPZnPcOCH）、Zn(II) 2,9,16,23-四（4-吡啶氧基）酞菁（SiNPZnPcOPy）和 Zn(II) 2,9,16,23-四（叔丁基）酞菁（SiNPZnPctBu），是在 AOT/1-丁醇/水胶束的非极性核中通过三乙氧基乙烯基硅烷和 3-氨丙基三乙氧基硅烷合成的。这些 SiNPs-Pc 的平均直径约为 20-25nm。紫外-可见吸收光谱呈现出嵌入纳米粒子的酞菁的特征 Soret 和 Q 带。此外，在水中检测到带有酞菁的 SiNPs 的红色荧光发射。SiNPs-Pc 产生了 2,2'-(蒽-9,10-二基)双(甲基丙二酸)的光解，这被用来检测水中单重态氧 O(Δ)的产生。此外，在存在 NADH 的情况下，通过硝基蓝四唑还原检测到超氧阴离子自由基的形成。在金黄色葡萄球菌和白色念珠菌中研究了微生物的光灭活。体外实验表明，SiNPZnPcOCH 和 SiNPZnPctBu 敏化诱导的光灭活随着辐照时间的增加而增强。在 30min 照射后，金黄色葡萄球菌的对数减少超过 7。同样，在 60min 照射后，这些 SiNPs-Pc 使白色念珠菌减少了 2.5 个对数。在这两种情况下，SiNPZnPcOPy 的光灭活活性都较低。光动力作用机制的研究表明，在叠氮化钠和二氮杂二环[2.2.2]辛烷存在的情况下，微生物细胞的光杀伤受到保护。此外，加入 D-甘露醇可降低细胞光损伤。因此，SiNPZnPcOCH 和 SiNPZnPctBu 在微生物细胞中的光动力活性是由 I 型和 II 型光氧化机制的共同贡献介导的。因此，硅纳米粒子是将 ZnPcOCH 和 ZnPctBu 载运到水溶液中以光杀灭微生物的有趣材料。
