[废水与垃圾渗滤液中抗生素抗性基因的比较研究]
[Comparative Investigation of Antibotic Resistance Genes Between Wastewater and Landfill Leachate].
作者信息
Huang Fu-Yi, Li Hu, An Xin-Li, Ouyang Wei-Ying, Su Jian-Qiang
机构信息
Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China.
University of Chinese Academy of Sciences, Beijing 100049, China.
出版信息
Huan Jing Ke Xue. 2016 Oct 8;37(10):3949-3954. doi: 10.13227/j.hjkx.2016.10.037.
The increasing output of wastewater and landfill leachate during the rapid urbanization exacerbated the human health risks induced by antibiotic resistance genes(ARGs). For further insight into antibiotic resistance genes(ARGs) of wastewater (adding leachate before treatment) and landfill leachate, high-throughput qPCR technique was used to investigate their abundance and diversities. A total of 187 unique ARGs were detected in wastewater, which was significantly higher than that in leachate (39 unique ARGs were detected)(<0.05). A total of 119 antibiotic resistance genes were significantly enriched in wastewater compared with the landfill leachate (<0.05), especially the -04 and , which belongs to mobile gene elements(MGEs) and -Lactamase, increased by 3338-fold and 1061-fold, respectively. Our results showed that the profile of ARGs was significantly different between the wastewater and leachate, and the wastewater was the important reservoir of ARGs.
快速城市化进程中废水和垃圾渗滤液产量的增加加剧了抗生素抗性基因（ARGs）对人类健康的风险。为了进一步了解废水（处理前添加渗滤液）和垃圾渗滤液中的抗生素抗性基因（ARGs），采用高通量qPCR技术研究了它们的丰度和多样性。废水中共检测到187种独特的ARGs，显著高于渗滤液中的检测结果（检测到39种独特的ARGs）（P<0.05）。与垃圾渗滤液相比，废水中共有119种抗生素抗性基因显著富集（P<0.05），特别是属于移动基因元件（MGEs）的intI1和blaTEM，分别增加了3338倍和1061倍。我们的结果表明，废水和渗滤液中ARGs的分布存在显著差异，废水是ARGs的重要储存库。
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