Wang Zhen-Hong, Zhang Han-Peng, Luo Zhuan-Xi
Fujian Province Key Laboratory of Modern Analytical Science and Separation Technology, College of Chemistry and Environment and Fujian Province, Minnan Normal University, Zhangzhou 363000, China.
Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China.
Huan Jing Ke Xue. 2016 Jul 8;37(7):2570-2576. doi: 10.13227/j.hjkx.2016.07.020.
To better understand and then to predict the ecological risk of arsenic influenced by phosphorus regimes in freshwater environment, the growth differences of () as well as its responses to the toxic stress of arsenate [As(Ⅴ)]were investigated under orthophosphate (DIP) and dissolved organic phosphorous (DOP) [adenosine triphosphate (ATP-P) and -sodium glycerophosphate (-P)] culture conditions. The results showed that grew and proliferated without any differences under DOP and DIP conditions during the first 5 days, while slower growth rates were observed in DOP conditions. Herein, the cell optical density () of in -P and ATP-P conditions was 78.0% and 75.4% of that under DIP condition respectively on the 7 day. The negative correlations between actual quantum yield (Yield) and Chlorophyll a (Chl-a) and indicated that Yield should not be used as a stable index to reflect the nutrition conditions of algae. However, Yield was a sensitive index to exactly represent the responses of to As(Ⅴ) toxicity, which showed significant differences under different phosphorus regimes. Obtained by , Yield and Chl-a, the 96h EC was in the order of DIP > -P > ATP-P. The similar toxic tolerant abilities to As(Ⅴ) of under -P and ATP-P conditions were lower than that of EC under DIP conditions by one to five orders of magnitude.
为了更好地理解并预测淡水环境中磷形态对砷生态风险的影响，研究了在正磷酸盐（溶解性无机磷，DIP）和溶解性有机磷（DOP，包括三磷酸腺苷磷（ATP-P）和甘油磷酸钠（-P））培养条件下，（）的生长差异及其对砷酸盐[As(Ⅴ)]毒性胁迫的响应。结果表明，在前5天，（）在DOP和DIP条件下生长和增殖无差异，但在DOP条件下生长速率较慢。在此，在第7天，-P和ATP-P条件下（）的细胞光密度（）分别为DIP条件下的78.0%和75.4%。实际量子产量（Yield）与叶绿素a（Chl-a）和（）之间的负相关表明，Yield不应作为反映藻类营养状况的稳定指标。然而，Yield是准确表征（）对As(Ⅴ)毒性响应的敏感指标，在不同磷形态下表现出显著差异。通过（）、Yield和Chl-a得到的96h EC50顺序为DIP > -P > ATP-P。-P和ATP-P条件下（）对As(Ⅴ)的类似耐毒能力比DIP条件下的EC50低1至5个数量级。
