Meng Xiang-Bin, Li Meng-Zhe, Li Hong-Tao, Gao Dong-Mei, Qi Jian-Hua
Key Laboratory of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, China.
Huan Jing Ke Xue. 2016 Nov 8;37(11):4147-4155. doi: 10.13227/j.hjkx.201605074.
In order to study the level and size distribution of microbial activity of atmospheric bioaerosols, bioaerosol samples were collected from November 2015 to January 2016 using size-fractionated bioaerosol samplers at the coastal region of Qingdao, and the microbial activity was measured using the fluorescein diacetate (FDA) hydrolysis method. The results showed that the level of microbial activity was in the range of 21.89-108.59 ng·m sodium fluorescein during the sampling period, with an average of 59.43 ng·m sodium fluorescein in Qingdao. Size distribution of microbial activity exhibited a tendency, the activity increased with increasing particle size. The microbial activity on particles with coarse size (>2.1 μm) was higher than that on fine size, with the highest average proportion of 24.06% for coarse size larger than 7.0 μm. The daily variation of microbial activity was different for different samples, which showed no significant diurnal variation in winter. The correlation analysis showed that microbial activity was significantly correlated with wind velocity(=0.445, =33, <0.01) during the sampling period. However, microbial activity showed no significant correlation with the meteorological factors, such as temperature,relative humidity and UV intensity during the sampling period. Moreover, there was no significant correlation of microbial activity with air quality factors, such as AQI, PM, PM, CO, NO, O and SO. Source of air mass had significant impact on microbial activity. The average level of microbial activity was 100.33 ng·m sodium fluorescein on sunny days, and the level decreased to 56.53 ng·m sodium fluorescein on hazy days. When the haze was mixed with fog and this special circumstance lasted for several days, the microbial activity reduced to 37.7% of the level of sunny days. Therefore, consecutive hazy weather had great influence on microbial activity.
为研究大气生物气溶胶的微生物活性水平及粒径分布，于2015年11月至2016年1月在青岛沿海地区使用分级生物气溶胶采样器采集生物气溶胶样本，并采用荧光素二乙酸酯（FDA）水解法测定微生物活性。结果表明，采样期间微生物活性水平在21.89 - 108.59 ng·m荧光素钠范围内，青岛地区平均为59.43 ng·m荧光素钠。微生物活性的粒径分布呈现一定趋势，活性随粒径增大而增加。粗粒径（>2.1μm）颗粒上的微生物活性高于细粒径颗粒，其中粒径大于7.0μm的粗粒径颗粒平均占比最高，为24.06%。不同样本的微生物活性日变化各异，冬季无明显昼夜变化。相关性分析表明，采样期间微生物活性与风速显著相关（=0.445，=33，<0.01）。然而，采样期间微生物活性与温度、相对湿度和紫外线强度等气象因素无显著相关性。此外，微生物活性与空气质量因子如空气质量指数（AQI）、PM、PM、CO、NO、O和SO也无显著相关性。气团来源对微生物活性有显著影响。晴天时微生物活性平均水平为100.33 ng·m荧光素钠， haze天降至56.53 ng·m荧光素钠。当 haze与雾混合且这种特殊情况持续数天时，微生物活性降至晴天水平的37.7%。因此，连续的 haze天气对微生物活性有很大影响。
