Yu Li-Fang, Du Qian-Qian, Fu Xue-Tao, Zhang Ru, Li Wen-Jiang, Peng Dang-Cong
Key Laboratory of Northwest Water Resources, Environment and Ecology of Ministry of Education, Xi'an University of Architecture and Technology, Xi'an 710055, China.
Huan Jing Ke Xue. 2016 Nov 8;37(11):4366-4371. doi: 10.13227/j.hjkx.201605026.
The communities and activity of nitrifiers collected from the raw sewage of the 2 and 3 wastewater treatment plants (WWTP) in Xi'an were investigated. FISH results indicated that the (AOB+NOB)/EUB percentages were (5.35±2.1)% and(6.0±2.8)% in the 2 and 3 WWTP, respectively. The dominant AOB was lineage and the dominant NOB was , the sub-dominant NOB was , and coexisted with . Respirometric assays showed that the influent nitrifiers were active following a 2-16 hour period of metabolic induction. The ammonium utilized rate was (0.32±0.12) mg·(L·h) and (0.43±0.17) mg·(L·h), nitrite utilized rate was (0.71±0.18) mg·(L·h) and (0.58±0.27) mg·(L·h) for nitrifers in raw sewage fed to the 2 and 3 WWTP, respectively. Therefore, nitrifiers were present and active in the raw sewage, and played the role of natural continuous seeding in the activated sludge system. Based on the nitrification activity, the estimated continuous seeding intensity of AOB and NOB was 0.08-0.09 g·(g·d) and 0.11-0.24 g·(g·d), respectively.
对取自西安第2和第3污水处理厂（WWTP）原污水中硝化细菌的群落和活性进行了研究。荧光原位杂交（FISH）结果表明，第2和第3污水处理厂中（氨氧化细菌+亚硝酸盐氧化细菌）/真细菌的百分比分别为（5.35±2.1）%和（6.0±2.8）%。优势氨氧化细菌为 菌系，优势亚硝酸盐氧化细菌为 ，次优势亚硝酸盐氧化细菌为 ，且与 共存。呼吸测定表明，经过2 - 16小时的代谢诱导后，进水硝化细菌具有活性。输入第2和第3污水处理厂的原污水中硝化细菌的铵利用率分别为（0.32±0.12）mg·（L·h）和（0.43±0.17）mg·（L·h），亚硝酸盐利用率分别为（0.71±0.18）mg·（L·h）和（0.58±0.27）mg·（L·h）。因此，硝化细菌存在于原污水中且具有活性，并在活性污泥系统中起到自然连续接种的作用。基于硝化活性，氨氧化细菌和亚硝酸盐氧化细菌的估计连续接种强度分别为0.08 - 0.09 g·（g·d）和0.11 - 0.24 g·（g·d）。
