[Optimization for Quantitative Processing of Fluorescence Hybridization (FISH)].

Precise quantification of , which is identified as a dominated filamentous bacterium of bulking sludge in the worldwide, is essential for bulking investigation and related control strategies. However, quantitative processing based on fluorescence hybridization (FISH) is prone to interference due to the specific characteristics of (hydrophobic surface with thick cell wall). Our study focused on pretreatment and process optimization to show that the proportion of was increased from 1.12% to 96.70% benefited by lysozyme (36000 U·mL), high probe concentration (4.5 ng·μL) and longer hybridization time (4 h) employed, mapping with the results of q-PCR method and Eikelboom & Jenkins Observation.