Zhang Ya-Jie, Li Ke, Zhu Hao-Ran, Zhang Hong-Xun
College of Resources and Environment, University of Chinese Academy of Sciences, Beijing 101408, China.
Water Affairs Research Institute, North China University of Water Resources and Electric Power, Zhengzhou 450045, China.
Huan Jing Ke Xue. 2017 Aug 8;38(8):3319-3329. doi: 10.13227/j.hjkx.201612253.
Water samples were collected in summer, autumn, winter, and spring in Beihai Lake for the analysis of water quality and microbial community structure. It was found that the water quality in summer and autumn was worse than in winter and spring, and TN and TP were the most important factors affecting the water quality. The microbial community structure was investigated using MiSeq high-throughput sequencing. It was found that the phyla Cyanobacteria, Proteobacteria, Actinobacteria, and Bacteroidetes were abundant in all the samples, whereas their relative abundances differed among the four seasons. In summer and autumn, the microbial diversities were higher, and the phylum Cyanobacteria was most abundant, with the genus dominant. In winter, the phylum Proteobacteria was dominant. In spring, Actinobacteria and Bacteroidetes were abundant, accounting for 51.5%-64.3% of the bacterial community. The redundancy analysis (RDA) results showed that the microbial communities were mostly affected by water temperature in Beihai Lake. Overall, the water quality of Beihai Lake was qualified for the use of landscape water. However, the abundance of in summer may lead to algal blooms. Thus, it is necessary to strengthen the monitoring and estimation of water quality in Beihai Lake.
于北海湖春夏秋冬四季采集水样，用于水质及微生物群落结构分析。结果发现，夏秋季节水质较冬春季节差，总氮（TN）和总磷（TP）是影响水质的最重要因素。采用MiSeq高通量测序技术对微生物群落结构进行研究。结果发现，所有样本中蓝藻门、变形菌门、放线菌门和拟杆菌门均占优势，但其相对丰度在四季中有所不同。夏秋季节，微生物多样性较高，蓝藻门最为丰富， 属占主导地位。冬季，变形菌门占主导地位。春季，放线菌门和拟杆菌门较为丰富，占细菌群落的51.5% - 64.3%。冗余分析（RDA）结果表明，北海湖微生物群落主要受水温影响。总体而言，北海湖水质符合景观用水标准。然而，夏季 的丰度可能导致藻类大量繁殖。因此，有必要加强对北海湖水质的监测和评估。
