Yang Ping-Ping, Yin Hua, Peng Hui, Tang Shao-Yu, Lu Miao, Liu Hao
Key Laboratory of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, Guangdong Provincial Engineering and Technology Research Center for Environmental Risk Prevention and Emergency Disposal, School of Environment and Energy, South China University of Technology, Guangzhou 510006, China.
Department of Chemistry, Jinan University, Guangzhou 510632, China.
Huan Jing Ke Xue. 2017 Aug 8;38(8):3536-3543. doi: 10.13227/j.hjkx.201702176.
In this study, municipal sludge, sawdust, and mushroom residues were used as raw materials for composting, and thermophiles and white-rot fungi were added into the compost in stages. By measuring physicochemical factors, including temperature, pH value, organic matter, water-soluble organic carbon, moisture content, total nitrogen, NH-N, NO-N, and germination index during the composting process, the effect of exogenous bacteria inoculation on the efficiency of compost was determined. By means of high-throughput sequencing technology, the variation of bacterial community structure and the impact of exogenous bacteria inoculation on bacterial community structure during sludge composting were also investigated. The results showed that the inoculation extended the high temperature duration, decreased the nitrogen loss, and accelerated the decomposition and detoxification of the compost. During the entire period of composting, the structure of bacterial community changed significantly. There was low similarity of bacterial community structure among different stages of the same composting, but high similarity was observed in different composting of the same period. The inoculation of thermophilic bacteria improved the abundance of bacterial community and increased the proportions of dominant genera in thermophilic phase, but changed no species of dominant genera. Canonical correspondence analysis showed that pH had the highest influence on the structure of bacterial community and that temperature possessed positive correlation with nine genera.
在本研究中，城市污泥、锯末和蘑菇渣被用作堆肥的原料，并分阶段向堆肥中添加嗜热菌和白腐真菌。通过测定堆肥过程中的温度、pH值、有机质、水溶性有机碳、水分含量、总氮、NH-N、NO-N和发芽指数等理化因子，确定了外源细菌接种对堆肥效率的影响。借助高通量测序技术，还研究了污泥堆肥过程中细菌群落结构的变化以及外源细菌接种对细菌群落结构的影响。结果表明，接种延长了高温持续时间，减少了氮素损失，加速了堆肥的分解和解毒。在整个堆肥期间，细菌群落结构发生了显著变化。同一堆肥不同阶段的细菌群落结构相似度较低，但同一时期不同堆肥之间的相似度较高。嗜热菌的接种提高了细菌群落的丰度，增加了嗜热阶段优势属的比例，但优势属的种类没有变化。典范对应分析表明，pH对细菌群落结构的影响最大，温度与9个属呈正相关。
