[Effects of Environmental Factors on the Synergy of Functional Bacteria in Completely Autotrophic Granular Sludge].

To obtain experimental evidences for optimizing a completely autotrophic nitrogen removal process based on granules, the effects of dissolved oxygen (DO) concentration, temperature (), initial ammonium (NH-N) concentration, and solution pH conditions on the synergy between the aerobic and anaerobic ammonium-oxidizing bacteria (AOB and AMX) were investigated using a single factor batch experiment, while the analysis of the microbial community structure within them was conducted using MiSeq high-throughput pyrosequencing. Results revealed that AOB (genus ) and AMX (genus ) dominated in the granules, representing relative abundances of 32.9% and 9.8%, respectively. For the granules, the highest specific nitrogen removal rate of (TN)=(17.7±1.0) mg·(g·h) was obtained at a DO concentration of 2 mg·L, while the initial NH-N concentration was set at 100 mg·L. And a lower DO level resulted in partial nitritation became the rate-limiting step of process, otherwise, it would be the ANAMMOX reaction instead. According to the free energy of the reactions, the activity of AMX was more sensitive to low temperature than that of AOB. When the reaction temperature was lower than 30℃, nitrite accumulation could be observed in bulk liquid, with the significant decrease of (TN) for the granules. Under the same oxygen supply conditions, an initial NH-N concentration lower than 100 mg·L could inhibit the activity of AMX partly. However, with an initial NH-N concentration over 150 mg·L, either oxygen-limiting or high free ammonia concentration could lead to the dramatic decrease of (TN). In addition, the effective synergy of the two types of ammonium oxidizers in granules was always achieved at solution pH in the range of 7.0-8.5.