Wang Han, Fang Fang, Li Kai, Xing Hui, Guo Jin-Song, Chen You-Peng, Zeng Qian-Song
College of Urban Construction and Environmental Engineering, Chongqing University, Chongqing 400045, China.
Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, China.
Huan Jing Ke Xue. 2017 Jan 8;38(1):238-246. doi: 10.13227/j.hjkx.201607221.
Intact ladderane lipids are a special kind of phospholipids in the anammoxosome membrane of AnAOB.Phospholipid organic mixture was extracted respectively from four sludge samples in anaerobic EGSB reactor,aerobic SBR reactor,autotrophic nitrogen removal SBBR reactor and anaerobic ammonium oxidation EGSB reactor.Phospholipid extracts of sludge were detected by the HPLC-ESI-MS/MS method to determine the types of intact ladderane lipids,and the specificity of intact ladderane lipids was also analyzed,at the same time,the bacterial community structure of autotrophic nitrogen removal SBBR reactor and anaerobic ammonium oxidation EGSB reactor was determined by high-throughput sequencing technology.Analytical results of intact ladderane lipids showed that intact ladderane lipids were not detected from anaerobic EGSB reactor and aerobic SBR reactor,and were detected from autotrophic nitrogen removal SBBR reactor and anaerobic ammonium oxidation EGSB reactor.Five kinds of intact ladderane lipids were detected in the phospholipid extract of sludge from autotrophic nitrogen removal SBBR reactor,and seven kinds of intact ladderane lipids were detected in the phospholipid extract of sludge from anaerobic ammonium oxidation EGSB reactor.The results of high-throughput sequencing confirmed that the autotrophic nitrogen SBBR reactor contained AnAOB of the genera Candidatus and Candidatus ,and the anaerobic ammonium oxidation EGSB reactor contained AnAOB of the genera Candidatus and Candidatus .These results illustrated that intact ladderane lipids were the peculiar phospholipids in AnAOB.
完整的梯形烷脂质是厌氧氨氧化菌（AnAOB）厌氧氨氧化体膜中的一种特殊磷脂。分别从厌氧膨胀颗粒污泥床（EGSB）反应器、好氧序批式反应器（SBR）、自养脱氮序批式生物膜反应器（SBBR）和厌氧氨氧化EGSB反应器的四种污泥样品中提取磷脂有机混合物。采用高效液相色谱-电喷雾串联质谱（HPLC-ESI-MS/MS）法检测污泥的磷脂提取物，以确定完整梯形烷脂质的类型，并分析完整梯形烷脂质的特异性，同时采用高通量测序技术测定自养脱氮SBBR反应器和厌氧氨氧化EGSB反应器的细菌群落结构。完整梯形烷脂质的分析结果表明，在厌氧EGSB反应器和好氧SBR反应器中未检测到完整的梯形烷脂质，而在自养脱氮SBBR反应器和厌氧氨氧化EGSB反应器中检测到了完整的梯形烷脂质。在自养脱氮SBBR反应器污泥的磷脂提取物中检测到五种完整的梯形烷脂质，在厌氧氨氧化EGSB反应器污泥的磷脂提取物中检测到七种完整的梯形烷脂质。高通量测序结果证实，自养脱氮SBBR反应器中含有“andidatus”属和“andidatus”属的厌氧氨氧化菌，厌氧氨氧化EGSB反应器中含有“andidatus”属和“andidatus”属的厌氧氨氧化菌。这些结果表明，完整的梯形烷脂质是厌氧氨氧化菌特有的磷脂。
