Qin Hong, Ren Qing-Shui, Yang Wen-Hang, Li Chang-Xiao
Key Laboratory of Eco-Environments in the Three Gorges Reservoir Region(Ministry of Education), School of Life Sciences, Southwest University, Chongqing 400715, China.
Huan Jing Ke Xue. 2017 May 8;38(5):2065-2073. doi: 10.13227/j.hjkx.201609072.
This study aimed to understand the soil actinobacterial community diversity in the urban and rural hydro-fluctuation zone of the Three Gorges Reservoir region. The hydro-fluctuation zone of Chongqing Great Theatre in Jiangbei District and the Ruxi river basin in Zhong County, Chongqing, was chosen as the study area. Soil samples were collected in June 2015, to measure actinobacterial biodiversity using the terminal-restriction fragment length polymorphism (T-RFLP) molecular method. Results showed that:1 The actinobacterial biodiversity was different in the urban and rural hydro-fluctuation zone after artificial vegetation restoration. The actinobacterial biodiversity of the urban hydro-fluctuation zone was significantly higher than that of the rural hydro-fluctuation zone (<0.05), which was most obvious in the surface soil. 2 The actinobacterial biodiversity of woodland was higher than that of grassland, but there was no significant difference in the actinobacterial biodiversity among the three soil layers in both the urban and rural hydro-fluctuation zone. 3 The dominant species of actinomycetes was different in different samples. 4 Redundancy analysis and the Monte-Carlo test revealed that water content and available nitrogen (AN) played a critical role in influencing actinobacterial biodiversity in the urban hydro-fluctuation zone (<0.05). In contrast, the water content, organic matter (OM), available nitrogen (AN) and available phosphorus (AP) played a critical role in influencing actinobacterial biodiversity in the rural hydro-fluctuation zone of the Three Gorges Reservoir region (<0.05). Thus, we concluded that the actinobacterial biodiversity of the urban hydro-fluctuation zone was significantly higher than that of the rural hydro-fluctuation zone in the Three Gorges Reservoir region, and such a result was attributed to the increased human disturbance and soil nutrients.
本研究旨在了解三峡库区城乡消落带土壤放线菌群落多样性。选取重庆市江北区重庆大剧院消落带及重庆市忠县汝溪河小流域作为研究区域。于2015年6月采集土壤样本，采用末端限制性片段长度多态性（T-RFLP）分子方法测定放线菌生物多样性。结果表明：1人工植被恢复后，城乡消落带放线菌生物多样性存在差异。城市消落带放线菌生物多样性显著高于农村消落带（<0.05），在表层土壤中最为明显。2林地放线菌生物多样性高于草地，但城乡消落带三个土层的放线菌生物多样性均无显著差异。3不同样本中放线菌优势种不同。4冗余分析和蒙特卡洛检验表明，含水量和有效氮（AN）对城市消落带放线菌生物多样性的影响显著（<0.05）。相比之下，含水量、有机质（OM）、有效氮（AN）和有效磷（AP）对三峡库区农村消落带放线菌生物多样性的影响显著（<0.05）。因此，我们得出结论，三峡库区城市消落带放线菌生物多样性显著高于农村消落带，这一结果归因于人类干扰和土壤养分的增加。
