Zhang Qiu-Ya, Ma Xiao-Yan, Wang Xiao-Chang, You Meng
School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China.
Huan Jing Ke Xue. 2017 Apr 8;38(4):1506-1512. doi: 10.13227/j.hjkx.201607232.
Targeting at the characteristics of low concentration but high risk of endocrine disrupting compounds in the aquatic environment, the current study investigated the distribution of estrogenicity and E2 level using recombinant yeast estrogen screen (YES) and enzyme linked immunosorbent assay (ELSIA) during domestic wastewater treatment processes. The results showed that the levels of estrogenicity and E2 in influents of wastewater treatment plants were 4.35-7.58 ng·L and 36.95-83.43 ng·L, respectively. It was found that biological treatment processes played a crucial role in the removal of estrogenicity and E2, such as oxidation ditch, A/O and A/O followed by MBR, where the removal of estrogenicity and E2 was 71.10%-75.54% and 75.88%-80.72%, respectively. The activated sludge, with an estrogencity level of 1.84-2.43 ng·g and an E2 level of 8.45-12.84 ng·g, had the function of eliminating estrogenic effect to a certain extent by adsorption. The estrogenic activity in the effluent of domestic wastewater was 1.06-2.19 ng·L, which will pose potential risk to the aquatic life in the environment. Last but not least, ELISA had a good correlation with YES assay, which provides a new method for rapid screening and assessment of water environmental estrogen.
针对水环境中内分泌干扰化合物浓度低但风险高的特点,本研究采用重组酵母雌激素筛选法(YES)和酶联免疫吸附测定法(ELSIA)研究了生活污水处理过程中雌激素活性和雌二醇(E2)水平的分布。结果表明,污水处理厂进水的雌激素活性和E2水平分别为4.35 - 7.58 ng·L和36.95 - 83.43 ng·L。研究发现,生物处理工艺在去除雌激素活性和E2方面起着关键作用,如氧化沟、A/O以及A/O后接膜生物反应器(MBR)工艺,其中雌激素活性和E2的去除率分别为71.10% - 75.54%和75.88% - 80.72%。活性污泥的雌激素活性水平为1.84 - 2.43 ng·g,E2水平为8.45 - 12.84 ng·g,具有一定的吸附消除雌激素效应的功能。生活污水出水的雌激素活性为1.06 - 2.19 ng·L,这将对环境中的水生生物构成潜在风险。最后但同样重要的是,酶联免疫吸附测定法(ELISA)与重组酵母雌激素筛选法(YES)具有良好的相关性,为水环境雌激素的快速筛选和评估提供了一种新方法。